Human GLO1 ELISA Kit PicoKine®

Glyoxalase I ELISA kit for Human

Human GLO1 Kit PicoKine™ (96 Tests). Quantitate Human GLO1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2209
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human GLO1 ELISA Kit PicoKine®

View all Glyoxalase I ELISA kits

SKU/Catalog Number

EK2209

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GLO1 Kit PicoKine™ (96 Tests). Quantitate Human GLO1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GLO1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2209)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-M184

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2209 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2209 is reactive to GLO1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2209 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Glyoxalase I

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Kit Components

Catalog Number Description Quantity
EK2209-CAP Anti-Human GLO1 Pre-coated 96-well strip microplate 1
EK2209-ST Human GLO1 Standard 2 vials, 10 ng/tube
EK2209-DA Human GLO1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01563126251,2502,5005,00010,000
O.D.0.0270.0420.0860.2560.5231.0681.3751.962

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Glyoxalase I, Dilution ratio of 1:1, concentration in serum and plasma is around 4 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human GLO1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3501269456537813664475
Standard deviation19.2565.99164.3419.2861.47214.8
CV(%)5.5%5.2%3.6%5.1%4.5%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Glyoxalase I in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135036537036836318.535.1%
Sample 21269130512881283128646.313.6%
Sample 345654541472046904629282.376.1%
*number of samples for each test n=16.

Gene/Protein Information For GLO1 (Source: Uniprot.Org, NCBI)

Gene Name

GLO1

Full Name

Lactoylglutathione lyase

Weight

20.778kDa

Superfamily

glyoxalase I family

Alternative Names

Aldoketomutase; EC 4.4.1.5; GLO1; GLOD1; Glx I; GLYI; glyoxalase domain containing 1; Glyoxalase I; glyoxalase Ialdoketomutase; Ketone-aldehyde mutase; lactoyl glutathione lyase; lactoylglutathione lyase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase GLO1 GLOD1, GLYI, HEL-S-74 glyoxalase I lactoylglutathione lyase|S-D-lactoylglutathione methylglyoxal lyase|aldoketomutase|epididymis secretory protein Li 74|glx I|glyoxalase domain containing 1|ketone-aldehyde mutase|lactoyl glutathione lyase|methylglyoxalase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GLO1, check out the GLO1 Infographic

GLO1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GLO1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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