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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human IGFBP6 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human IGFBP6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IGFBP6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IGFBP6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0962)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A25-G240
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0962 is reactive to IGFBP6 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0962 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGFBP-6
Insulin-like growth factor-binding protein 6 (IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.
|EK0962-CAP||Anti-Human IGFBP6 Pre-coated 96-well strip microplate||1|
|EK0962-ST||Human IGFBP6 Standard||2 vials, 10 ng/tube|
|EK0962-DA||Human IGFBP6 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human IGFBP6 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IGFBP6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IGFBP-6 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For IGFBP6 (Source: Uniprot.Org, NCBI)
Insulin-like growth factor-binding protein 6
IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6 IGFBP6 IBP6 insulin like growth factor binding protein 6 insulin-like growth factor-binding protein 6|IBP-6|IGF binding protein 6|IGFBP-6*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IGFBP6, check out the IGFBP6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGFBP6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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