This website uses cookies to ensure you get the best experience on our website.
Human IL-4 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-4. 96wells/kit, with removable strips. Cited in 29 publication(s).
Human IL-4 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0404 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <1.5pg/ml |
| Assay Range: | 15.6pg/ml-1000pg/ml |
Overview
| Product Name | Human IL-4 ELISA Kit PicoKine™
See all IL4 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0404 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human IL4 with <1.5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL4 tissue specificity: |
| Cite This Product | Human IL-4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0404) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <1.5pg/ml |
| Assay Range | 15.6pg/ml-1000pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: H25-S153 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | D4HNR6 |
|---|---|
| Gene Name | IL4 |
| Protein Name | Interleukin-4 |
| Subcellular Localization | Secreted . |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Human IL-4 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0404-CAP | 96-well plate precoated with anti-Human IL4 antibody | 1 |
| EK0404-ST | lyophilized recombinant Human IL4 standard | 10ng/tube |
| EK0404-DA | biotinylated anti-Human IL4 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0404 Human IL-4 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.019 | 0.042 | 0.067 | 0.129 | 0.339 | 0.780 | 1.651 | 2.241 |
We measured random samples of Human IL-4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 35 | 216 | 508 | 33 | 195 | 532 |
| Standard deviation | 2.03 | 12.52 | 21.33 | 2.04 | 11.89 | 29.26 |
| CV(%) | 5.8% | 5.8% | 4.2% | 6.2% | 6.1% | 5.5% |
We measured the performance consistency of Human IL-4 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 31 | 34 | 34 | 33 | 1.5 | 4.5% |
| Sample 2 | 216 | 213 | 203 | 197 | 207 | 7.62 | 3.6% |
| Sample 3 | 508 | 515 | 448 | 502 | 493 | 26.52 | 5.3% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 29
| Li L, Yu Ch, Ying Hz, Yu Jm. J Ethnopharmacol. 2013 May 2;147(1):238-44. Doi: 10.1016/J.Jep.2013.03.012. Epub 2013 Mar 15. Antiviral Effects Of Modified Dingchuan Decoction Against Respiratory Syncytial Virus Infection In Vitro And In An Immunosup... | PubMed ID 23506991 |
| Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ... | PubMed ID 23423691 |
| Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu... | PubMed ID 22464914 |
| Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (???????????? | PubMed ID 25428340 |
| Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro. | PubMed ID 20609400 |
| Li S, Hu X, Wang Z, Wu M, Zhang J. Inflamm Res. 2015 May;64(5):363-71. Doi: 10.1007/S00011-015-0816-Y. Epub 2015 Mar 27. Different Profiles Of Notch Signaling In Cigarette Smoke-Induced Pulmonary Emphysema And Bleomycin-Induced Pulmonary Fibrosis. | PubMed ID 25813392 |
| Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The... | PubMed ID 25744647 |
| Huang J, Wang P, Li M, Ge J, Chen J, Chen X. Int J Mol Med. 2015 Aug;36(2):493-500. Doi: 10.3892/Ijmm.2015.2249. Epub 2015 Jun 16. Trichostatin A Reduces Cisplatin-Induced Ototoxicity Through The Stat6 Signaling Pathway. | PubMed ID 26080623 |
| Wang Xj, Deng Hz, Jiang B, Yao H. Int J Colorectal Dis. 2012 May;27(5):575-81. Doi: 10.1007/S00384-011-1352-Z. Epub 2011 Nov 15. The Natural Plant Product Sophocarpine Ameliorates Dextran Sodium Sulfate-Induced Colitis In Mice By Regulating Cytoki... | PubMed ID 22083209 |
| Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert... | PubMed ID 24368037 |
| Qiu Jl, Zhu Wl, Lu Yj, Bai Zf, Liu Zg, Zhao P, Sun C, Zhang Yb, Li H, Liu W. Neurochem Int. 2015 Jun-Jul;85-86:46-52. Doi: 10.1016/J.Neuint.2015.04.007. Epub 2015 May 4. The Selective Mglur5 Agonist Chpg Attenuates So2-Induced Oxidative Stress And... | PubMed ID 25953665 |
| Jin Q, Cheng J, Liu Y, Wu J, Wang X, Wei S, Zhou X, Qin Z, Jia J, Zhen X. Brain Behav Immun. 2014 Aug;40:131-42. Doi: 10.1016/J.Bbi.2014.03.003. Epub 2014 Mar 12. Improvement Of Functional Recovery By Chronic Metformin Treatment Is Associated With... | PubMed ID 24632338 |
| Ou J, Shi W, Xu Y, Tao Z. Ann Allergy Asthma Immunol. 2014 Dec;113(6):658-665.E1. Doi: 10.1016/J.Anai.2014.08.015. Epub 2014 Sep 17. Intranasal Immunization With Dna Vaccine Coexpressing Der P 1 And Ubiquitin In An Allergic Rhinitis Mouse Model. | PubMed ID 25240330 |
| Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model. | PubMed ID 24738018 |
| Li J, Li Ff, Zuo W, Zhou Y, Hao Hy, Dang J, Jiang M, He Mz, Deng Dr. J Huazhong Univ Sci Technolog Med Sci. 2014 Aug;34(4):586-90. Doi: 10.1007/S11596-014-1320-2. Epub 2014 Aug 19. Up-Regulated Expression Of Tim-3/Gal-9 At Maternal-Fetal Interface... | PubMed ID 25135732 |
| Berni Canani R, Paparo L, Nocerino R, Cosenza L, Pezzella V, Di Costanzo M, Capasso M, Del Monaco V, D'Argenio V, Greco L, Salvatore F. Clin Epigenetics. 2015 Mar 31;7(1):38. Doi: 10.1186/S13148-015-0070-8. Ecollection 2015. Differences In Dna Met... | PubMed ID 25859290 |
| Ozkara S, Keles E, Ilhan N, Gungor H, Kaygusuz I, Alpay Hc. Eur Arch Otorhinolaryngol. 2012 Dec;269(12):2519-24. Doi: 10.1007/S00405-012-1967-X. Epub 2012 Feb 23. The Relationship Between Th1/Th2 Balance And 1??,25-Dihydroxyvitamin D | PubMed ID 22358381 |
| Wang Yz, Feng Xg, Shi Qg, Hao Yl, Yang Y, Zhang Am, Kong Qx. Inflammation. 2013 Apr;36(2):337-45. Doi: 10.1007/S10753-012-9551-5. Silencing Of Mir155 Promotes The Production Of Inflammatory Mediators In Guillain-Barr?? Syndrome In Vitro. | PubMed ID 23065188 |
| Dang, W., Xie, W. & Zhou, J. (2017). Expression of PYCARD Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Primary Gout Patients with Different Chinese Medicine Syndromes. Chinese Journal of Integrative Medicine, 1-8. Advance ... | PubMed ID 29086221 |
| Liu B, Piao X, Guo L, Wang G, Sun W, Gao L, Zheng X, Fang Y. Evid Based Complement Alternat Med. 2017;2017:7323129. doi: 10.1155/2017/7323129. Epub 2017 Nov 20. A New Chinese Medicine Intestine Formula Greatly Improves the Effect of Aminosalicylat... | PubMed ID 29358969 |
| Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome | PubMed ID 27168826 |
| Capsaicin induces immunogenic cell death in human osteosarcoma cells | PubMed ID 27446273 |
| Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways | PubMed ID 30015892 |
| Association between IL-4 gene polymorphisms, IL-4 serum levels, and ankylosing spondylitis | PubMed ID 27813599 |
| IL-4, TGF-?, NF-? B and MPO levels in Patients with Treatment Resistant Schizophrenia | PubMed ID 27711937 |
| The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility | PubMed ID 29914245 |
| Association between IL-4 gene polymorphisms, IL-4 serum levels, and ankylosing spondylitis | PubMed ID 27813599 |
| IL-4, TGF-?, NF-? B and MPO levels in Patients with Treatment Resistant Schizophrenia | PubMed ID 27711937 |
| Effects of Electroconvulsive Therapy on Some Inflammatory Factors in Patients With Treatment-Resistant Schizophrenia | PubMed ID 26886746 |
Submit your review to [email protected] or to Biocompare.com for an Amazon giftcard.
0 reviews
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0404 Human IL-4 ELISA Kit PicoKine™
1 Related Questions
Order Product (EK0404)
Shipping Information
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
Contact Information
| [email protected] | |
| (888) 466-3604 |
Boster Guarantee
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.
See Details
In stock
Order Product
EK0404
$369.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.