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Product Info Summary
| SKU: | EK0779 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
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Product info
Product Name
Human IL-7/Interleukin-7 ELISA Kit PicoKine®
SKU/Catalog Number
EK0779
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-7/Interleukin-7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL7 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-7/Interleukin-7 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0779)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: D26-H177
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0779 is reactive to IL7 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
EK0779 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-7
IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7 (IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V (D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0779-CAP | Anti-Human IL7 Pre-coated 96-well strip microplate | 1 |
| EK0779-ST | Human IL7 Standard | 2 vials, 10 ng/tube |
| EK0779-DA | Human IL7 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.013 | 0.064 | 0.098 | 0.196 | 0.411 | 0.630 | 1.137 | 1.926 |
Data Example Images
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Human IL-7 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-7/Interleukin-7 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 17 | 81 | 209 | 15 | 78 | 192 |
| Standard deviation | 0.98 | 5.50 | 9.82 | 1.00 | 5.38 | 10.56 |
| CV (%) | 5.8% | 6.9% | 4.7% | 6.7% | 6.9% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-7 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 17 | 18 | 18 | 18 | 17 | 0.43 | 2.5% |
| Sample 2 | 81 | 89 | 83 | 75 | 82 | 5 | 6% |
| Sample 3 | 209 | 226 | 204 | 221 | 215 | 8.86 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For IL7 (Source: Uniprot.Org, NCBI)
Uniprot ID
P13232
Gene ID
3574
Gene Name
IL7
Full Name
Interleukin-7
Weight
20.187kDa
Superfamily
IL-7/IL-9 family
Alternative Names
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF IL7 IL-7 interleukin 7 interleukin-7
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL7, check out the IL7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human IL-7/Interleukin-7 ELISA Kit PicoKine® (EK0779)
EK0779 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
mohammed,R.J.,& Salih,L.A.(2021).The Correlation between Maternal Vitamin D and Interleukin-17 Levels and Fetal Biophysical Profile.Iraqi Journal of Science,62(6),1836-1842.https://doi.org/10.24996/ijs.2021.62.6.9
Species: Human
EK0779 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
mohammed,R.J.,& Salih,L.A.(2021).The Correlation between Maternal Vitamin D and Interleukin-17 Levels and Fetal Biophysical Profile.Iraqi Journal of Science,62(6),1836-1842.https://doi.org/10.24996/ijs.2021.62.6.9
Species: Human
EK0779 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
11 Customer Q&As for Human IL-7/Interleukin-7 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for IL-7 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-11-27
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-7 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-11-27
Question
Q: Can IL-7 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-11-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-11-16
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
B. Kapoor
Verified customer
Asked: 2019-08-25
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-7 using the EK0779 Human IL-7 Picokine® ELISA Kit?
C. Anand
Verified customer
Asked: 2019-07-04
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-07-04
Question
Do you know if cytokines can be detected accurately in placental tissue samples stored at room temperature in 70% ethanol using EK0779?
Verified customer
Asked: 2019-06-11
Answer
Our lab hasn't woked on placental tissue samples using Human IL-7 ELISA Kit PicoKine™ (EK0779). We are uncertain if cytokines can be detected accurately in tissue samples stored at room temperature in 70% ethanol. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-06-12
Question
Is there an existing protocol for placental tissue homogenization that has been optimized for use with EK0779?
Verified customer
Asked: 2019-06-11
Answer
Our lab hasn't woked on placental tissue samples using Human IL-7 ELISA Kit PicoKine™ (EK0779). We recommend to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit. Please see the following suggested protocol: Preparation of lysate from tissues 1. Mince tissue and then rinse tissue in ice-cold PBS. 2. Place minced tissue in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS(Volumes of PBS must be determined in relation to the amount of tissue present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no tissue chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 9. Aliquot supernatant and store at -20°C.
Boster Scientific Support
Answered: 2019-06-11
Question
Q: What is the optimal O.D. value for IL-7 ELISA kit? I performed your IL-7 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-7 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-7 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-7 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2018-08-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-02-17
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-02-17
Question
Q: can you recommend the dilution ratio of serum samples for detection of IL-7 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-7 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-09-06
Question
Q: how do I analyze ELISA data? I measured IL-7 level in serum.
K. Gonzalez
Verified customer
Asked: 2017-05-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-05-14
Question
I've recently purchased one of BosterBio's human IL-17 ELISA kits. I would like to use it to detect IL-17 levels in human placental tissue. Is there an existing protocol for placental tissue homogenization that has been optimized for use with this kit? Additionally, is it known if cytokines can be detected accurately in tissue samples stored at room temperature in 70% ethanol?
Verified Customer
Verified customer
Asked: 2016-09-30
Answer
Our lab hasn't woked on placental tissue samples using EK0779 human IL-17 ELISA kit. We are uncertain if
Boster Scientific Support
Answered: 2016-09-30
