Anti-Kallikrein 1/KLK1 Antibody Picoband®

Product Name

Anti-Kallikrein 1/KLK1 Antibody Picoband®

View all KLK1 Antibodies

SKU/Catalog Number

A02789

Size

100 μg/vial

Form

Lyophilized

Description

KLK1 (tissue kallikrein) is a serine protease (peptidase S1 family) that generates Lys-bradykinin by proteolysis of kininogen-1, placing it in kallikrein–kinin signaling relevant to vascular tone and cardiovascular phenotypes (model dependent).Assay context: human-reactive antibody validated for capture ELISA and WB with on-page starting dilutions (WB 0.1–0.5 µg/mL; ELISA Cap 1–5 µg/mL). Often contextualized with protease-inhibitor axes such as SERPINA1 (A1AT) and fibrinolysis regulators like SERPINF2 (α2-antiplasmin) when interpreting net proteolytic activity in plasma/tissue microenvironments (putative).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Kallikrein 1/KLK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02789)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Kallikrein 1 recombinant protein (Position: I25-S262).

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02789 is reactive to KLK1 in Human

Observed Molecular Weight

32 kDa

Calculated molecular weight

28.9 kDa

Background of KLK1

KLK1 (KALLIKREIN 1), also called KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. KLK1 is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The KLK1 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and its exact cytogenetic location is 19q13.33. The KLK1 gene contains 5 coding exons. And KLK1 is the most centromeric gene in the cluster. Mice lacking tissue kallikrein are unable to generate significant levels of kinins in most tissues and develop cardiovascular abnormalities early in adulthood despite normal blood pressure. The protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02789 is guaranteed for WB, ELISA (Cap) Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

See full target information KLK1

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