Anti-Kallikrein 1/KLK1 Antibody Picoband™

Product Name

Anti-Kallikrein 1/KLK1 Antibody Picoband™

View all Kallikrein 1 Antibodies

SKU/Catalog Number

A02789

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Kallikrein 1/KLK1 Antibody Picoband™ catalog # A02789. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Kallikrein 1/KLK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02789)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Kallikrein 1 recombinant protein (Position: I25-S262).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02789 is reactive to KLK1 in Human

Applications

A02789 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

32 kDa

Calculated molecular weight

28.89kDa

Background of Kallikrein 1

KLK1 (KALLIKREIN 1), also called KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. KLK1 is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The KLK1 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and its exact cytogenetic location is 19q13.33. The KLK1 gene contains 5 coding exons. And KLK1 is the most centromeric gene in the cluster. Mice lacking tissue kallikrein are unable to generate significant levels of kinins in most tissues and develop cardiovascular abnormalities early in adulthood despite normal blood pressure. The protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Kallikrein 1 using anti-Kallikrein 1 antibody (A02789).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human KLK1 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 1 antigen affinity purified polyclonal antibody (Catalog # A02789) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 1 at approximately 32KD. The expected band size for Kallikrein 1 is at 29KD.

Click image to see more details

Figure 2. Sandwich ELISA - Recombinant human Kallikrein 1/KLK1 protein standard curve.
Use in combination with reagents from Human Kallikrein 1/KLK1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0903).

Gene/Protein Information For KLK1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.21; EC 3.4.21.35; glandular kallikrein 1; hK1; Kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein serine protease 1; kallikrein-1; Kidney/pancreas/salivary gland kallikrein; KLK1; Klk6; KLKR; Tissue kallikrein KLK1 KLKR, Klk6, hK1 kallikrein 1 kallikrein-1|glandular kallikrein 1|kallikrein 1, renal/pancreas/salivary|kallikrein serine protease 1|kidney/pancreas/salivary gland kallikrein|tissue kallikrein

For more info on KLK1, check out the KLK1 Infographic

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In this infographic, you will see the following information for KLK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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