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Product Info Summary
| SKU: | EK1688 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Klotho ELISA Kit PicoKine®
SKU/Catalog Number
EK1688
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Klotho ELISA Kit PicoKine™ (96 Tests). Quantitate Human KL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Klotho ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1688)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E34-S981
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1688 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1688 is reactive to KL in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1688 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Klotho
Klotho (KL) is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss and alcohol consumption.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1688-CAP | Anti-Human KL Pre-coated 96-well strip microplate | 1 |
| EK1688-ST | Human KL Standard | 2 vials, 10 ng/tube |
| EK1688-DA | Human KL Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.056 | 0.119 | 0.161 | 0.245 | 0.448 | 0.765 | 1.261 | 1.941 |
Data Example Images
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Human Klotho PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Klotho, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 156 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Klotho ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 308 | 1224 | 5567 | 337 | 1220 | 5449 |
| Standard deviation | 15.4 | 79.56 | 239.38 | 20.55 | 102.48 | 321.49 |
| CV (%) | 5% | 6.5% | 4.3% | 6.1% | 8.4% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Klotho in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 308 | 313 | 332 | 326 | 319 | 9.65 | 3% |
| Sample 2 | 1224 | 1059 | 1107 | 1175 | 1141 | 63.09 | 5.5% |
| Sample 3 | 5567 | 6048 | 6003 | 5069 | 5671 | 395.46 | 6.9% |
Protein Target Info & Infographic
Gene/Protein Information For KL (Source: Uniprot.Org, NCBI)
Gene Name
KL
Full Name
Klotho
Weight
116.181kDa
Superfamily
glycosyl hydrolase 1 family
Alternative Names
EC 3.2.1; EC 3.2.1.31; KL; Klotho KL HFTC3 klotho klotho
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KL, check out the KL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Klotho ELISA Kit PicoKine® (EK1688)
Hello CJ!
EK1688 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Exercise-Linked Irisin Prevents Mortality and Enhances Cognition in a Mice Model of Cerebral Ischemia by Regulating Klotho Expression
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Human Klotho ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Klotho antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Klotho antigen?
J. Mangal
Verified customer
Asked: 2020-08-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-08-16
Question
Can this ELISA kit recognize alpha Klotho soluble and secreted protein?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
EK1688 can detect both alpha Klotho soluble and secreted proteins.
Boster Scientific Support
Answered: 2019-12-23
Question
Is it possible to get the antigen sequence for the protein against which the primary antibody was raised for EK1688?
Verified customer
Asked: 2019-10-31
Answer
For the Human Klotho ELISA Kit PicoKine™ (EK1688), the antigen sequence for the standard, capture antibody, and detection antibody is E34-S981, Uniprot Id Q9UEF7
Boster Scientific Support
Answered: 2019-10-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-10-01
Question
Q: how to proceed with the analysis of ELISA data? I have performed Klotho level in cell lysates.
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-21
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Klotho using the EK1688 Human Klotho Picokine® ELISA Kit?
Z. Williams
Verified customer
Asked: 2018-02-15
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-02-15
Question
Q: What is the optimal O.D. value for Klotho ELISA kit? I performed your Klotho ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Klotho even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2015-01-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Klotho ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of Klotho that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2015-01-27
