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Human MMP13 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP13. 96wells/kit, with removable strips. Cited in 11 publication(s).
Human MMP13 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0468 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, serum and plasma( heparin). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <5pg/ml |
| Assay Range: | 156pg/ml-10000pg/ml |
Overview
| Product Name | Human MMP13 ELISA Kit PicoKine™
See all MMP13 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0468 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human MMP13 with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MMP13 tissue specificity: Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue. . |
| Cite This Product | Human MMP13 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0468) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma( heparin). |
| Sensitivity | <5pg/ml |
| Assay Range | 156pg/ml-10000pg/ml |
| Immunogen | Expression system for standard: NSO; Immunogen sequence: L20-C471 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | A8K846 |
|---|---|
| Gene Name | MMP13 |
| Protein Name | Collagenase 3 |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix . Secreted . |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Human MMP13 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0468-CAP | 96-well plate precoated with anti-Human MMP13 antibody | 1 |
| EK0468-ST | lyophilized recombinant Human MMP13 standard | 10ng/tube |
| EK0468-DA | biotinylated anti-Human MMP13 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0468 Human MMP13 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10000 |
| O.D. | 0.121 | 0.230 | 0.347 | 0.542 | 0.838 | 1.249 | 1.770 | 2.157 |
We measured random samples of Human MMP13 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 219 | 1742 | 4482 | 203 | 1816 | 4413 |
| Standard deviation | 14.01 | 116.71 | 313.74 | 16.4%2 | 146.4%8 | 357.45 |
| CV(%) | 6.4% | 6.7% | 7% | 7.5% | 8% | 8.1% |
We measured the performance consistency of Human MMP13 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 219 | 230 | 199 | 236 | 221 | 14.08 | 6.3% |
| Sample 2 | 1742 | 1734 | 1789 | 1510 | 1693 | 108.14 | 6.3% |
| Sample 3 | 4482 | 4805 | 4585 | 4324 | 4549 | 174.6 | 3.8% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 11
| Capsoni F, Ongari Am, Lonati C, Accetta R, Gatti S, Catania A. Bmc Musculoskelet Disord. 2015 Jun 21;16:154. Doi: 10.1186/S12891-015-0615-1. ??-Melanocyte-Stimulating-Hormone (??-Msh) Modulates Human Chondrocyte Activation Induced By Proinflammato... | PubMed ID 26093672 |
| Nikniaz Z, Ostadrahimi A, Mahdavi R, Ebrahimi Aa, Nikniaz L. Complement Ther Med. 2014 Oct;22(5):864-9. Doi: 10.1016/J.Ctim.2014.07.004. Epub 2014 Jul 16. Effects Of Elaeagnus Angustifolia L. Supplementation On Serum Levels Of Inflammatory Cytokin... | PubMed ID 25440377 |
| Qin Y, He J, Xia L, Guo H, He C. Acupunct Med. 2013 Jun;31(2):214-21. Doi: 10.1136/Acupmed-2012-010289. Epub 2013 Mar 21. Effects Of Electro-Acupuncture On Oestrogen Levels, Body Weight, Articular Cartilage Histology And Mmp-13 Expression In Ovari... | PubMed ID 23519019 |
| Xu M, Zhang L, Zhao L, Gao S, Han R, Su D, Lei G. Rheumatol Int. 2013 May;33(5):1313-9. Doi: 10.1007/S00296-012-2548-4. Epub 2012 Nov 7. Phosphorylation Of Osteopontin In Osteoarthritis Degenerative Cartilage And Its Effect On Matrix Metalloprotea... | PubMed ID 23132541 |
| Yang B, Gao J, Rao Z, Shen Q. Scand J Clin Lab Invest. 2012 Oct;72(6):501-5. Doi: 10.3109/00365513.2012.699638. Epub 2012 Sep 5. Clinicopathological Significance And Prognostic Value Of Mmp-13 Expression In Colorectal Cancer. | PubMed ID 22950625 |
| Huang Gf, Zou J, Shi J, Zhang Dy, Pen Hf, Zhang Q, Gao Y, Wang By. Evid Based Complement Alternat Med. 2014;2014:731395. Doi: 10.1155/2014/731395. Epub 2014 May 27. The Effect Of Electroacupuncture On The Extracellular Matrix Synthesis And Degrada... | PubMed ID 24987434 |
| Xia L, Luo Ql, Lin Hd, Zhang Jl, Guo H, He Cq. Rheumatol Int. 2012 Sep;32(9):2847-56. Doi: 10.1007/S00296-011-2080-Y. Epub 2011 Sep 1. The Effect Of Different Treatment Time Of Millimeter Wave On Chondrocyte Apoptosis, Caspase-3, Caspase-8, And Mm... | PubMed ID 21881990 |
| Serum and saliva collagenase-3 (MMP-13) in patients with oral lichen planus and oral squamous cell carcinoma | PubMed ID 26478876 |
| A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1?-induced activation of AP-1 in human OA chondrocytes | PubMed ID 28256567 |
| Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma | PubMed ID 28749626 |
| Resveratrol inhibits the IL-1%u03B2-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and-independent %u2026 | PubMed ID 28204817 |
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Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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