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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma ( heparin).|
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Human MMP13/Collagenase 3 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MMP13 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP13 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human MMP13/Collagenase 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0468)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L20-C471
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0468 is reactive to MMP13 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin).
EK0468 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0468-CAP||Anti-Human MMP13 Pre-coated 96-well strip microplate||1|
|EK0468-ST||Human MMP13 Standard||2 vials, 10 ng/tube|
|EK0468-DA||Human MMP13 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MMP13 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP13/Collagenase 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MMP-13 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MMP13 (Source: Uniprot.Org, NCBI)
peptidase M10A family
CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC 184.108.40.206; EC 220.127.116.11; EC 18.104.22.168; EC 22.214.171.124; EC 126.96.36.199; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP13; MMP-13 MMP13 CLG3, MANDP1, MDST, MMP-13 matrix metallopeptidase 13 collagenase 3|matrix metalloproteinase 13 (collagenase 3)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MMP13, check out the MMP13 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0468 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Elevated synovial fluid IL-33 and IL-6 levels and cartilage degeneration in stage III osteonecrosis of the femoral head
Dopamine D2 receptor suppresses gastric cancer cell invasion and migration via inhibition of EGFR/AKT/MMP-13 pathway
HOXB5 promotes retinoblastoma cell migration and invasion via ERK1/2 pathway-mediated MMPs production
Anti-inflammatory and anti-arthritic effects of the ethanolic extract of Aralia continentalis Kitag. in IL-1β-stimulated human fibroblast-like synoviocytes and rodent models of polyarthritis and nociception
Wogonin, a plant derived small molecule, exerts potent anti-inflammatory and chondroprotective effects through the activation of ROS/ERK/Nrf2 signaling pathways in human Osteoarthritis chondrocytes
Resveratrol inhibits the IL-1%u03B2-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and-independent %u2026
Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma
A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1?-induced activation of AP-1 in human OA chondrocytes
Serum and saliva collagenase-3 (MMP-13) in patients with oral lichen planus and oral squamous cell carcinoma
Xia L, Luo Ql, Lin Hd, Zhang Jl, Guo H, He Cq. Rheumatol Int. 2012 Sep;32(9):2847-56. Doi: 10.1007/S00296-011-2080-Y. Epub 2011 Sep 1. The Effect Of Different Treatment Time Of Millimeter Wave On Chondrocyte Apoptosis, Caspase-3, Caspase-8, And Mm...
Huang Gf, Zou J, Shi J, Zhang Dy, Pen Hf, Zhang Q, Gao Y, Wang By. Evid Based Complement Alternat Med. 2014;2014:731395. Doi: 10.1155/2014/731395. Epub 2014 May 27. The Effect Of Electroacupuncture On The Extracellular Matrix Synthesis And Degrada...
Yang B, Gao J, Rao Z, Shen Q. Scand J Clin Lab Invest. 2012 Oct;72(6):501-5. Doi: 10.3109/00365513.2012.699638. Epub 2012 Sep 5. Clinicopathological Significance And Prognostic Value Of Mmp-13 Expression In Colorectal Cancer.
Xu M, Zhang L, Zhao L, Gao S, Han R, Su D, Lei G. Rheumatol Int. 2013 May;33(5):1313-9. Doi: 10.1007/S00296-012-2548-4. Epub 2012 Nov 7. Phosphorylation Of Osteopontin In Osteoarthritis Degenerative Cartilage And Its Effect On Matrix Metalloprotea...
Qin Y, He J, Xia L, Guo H, He C. Acupunct Med. 2013 Jun;31(2):214-21. Doi: 10.1136/Acupmed-2012-010289. Epub 2013 Mar 21. Effects Of Electro-Acupuncture On Oestrogen Levels, Body Weight, Articular Cartilage Histology And Mmp-13 Expression In Ovari...
Nikniaz Z, Ostadrahimi A, Mahdavi R, Ebrahimi Aa, Nikniaz L. Complement Ther Med. 2014 Oct;22(5):864-9. Doi: 10.1016/J.Ctim.2014.07.004. Epub 2014 Jul 16. Effects Of Elaeagnus Angustifolia L. Supplementation On Serum Levels Of Inflammatory Cytokin...
Capsoni F, Ongari Am, Lonati C, Accetta R, Gatti S, Catania A. Bmc Musculoskelet Disord. 2015 Jun 21;16:154. Doi: 10.1186/S12891-015-0615-1. ??-Melanocyte-Stimulating-Hormone (??-Msh) Modulates Human Chondrocyte Activation Induced By Proinflammato...
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