Human (mammalian cell expression) recombinant HMGB1 C23AC45AC106A protein, AF

Product Name

Human (mammalian cell expression) recombinant HMGB1 C23AC45AC106A protein, AF

View all HMGB1 recombinant proteins

SKU/Catalog Number

PROTP09429-5

Size

5ug,20ug,100ug

Tag

His-SUMO Tag (N-term)

Description

High Mobility Group protein B1 protein (HMGB1) is the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 25 kDa single chain polypeptide containing three domains: two N-terminal HMG boxes A and B, and a negatively charged 30 a.a. C-terminal region that contains only Asp and Glu. Post-translational modification on HMGB1 have been reported, affect its localization, receptor interactions, and function. HMGB1, with a disulfide bond between C23 and C45 , have been reported that cause cytokine production and the activation of NF-κB. Otherwise, the fully oxidized form has no immune function, losing its proinflammatory effect and the apoptotic cell death activation function. Here, we developed HMGB1 C23A, C45A & 10?A mutant proteins, the fully oxidized HMGB1, eliminant the disulfide bond formation.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Human (mammalian cell expression) recombinant HMGB1 C23AC45AC106A protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP09429-5)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Purity

>98% as determined by SDS-PAGE.

Predicted MW

24.9 kDa

Validation Images & Assay Conditions

See full target information HMGB1

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