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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human MANF ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MANF ELISA Kit PicoKine™ (96 Tests). Quantitate Human MANF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MANF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1845)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L25-L182
93.6 pg/ml - 6,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1845 is reactive to MANF in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1845 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MANF
MANF is a 20kDa protein which belongs to the ARMET family. This gene is mapped to 3p21.2. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.
|EK1845-CAP||Anti-Human MANF Pre-coated 96-well strip microplate||1|
|EK1845-ST||Human MANF Standard||2 vials, 10 ng/tube|
|EK1845-DA||Human MANF Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MANF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MANF, Dilution ratio of 1:5, concentration in serum is around 8ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MANF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MANF in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MANF (Source: Uniprot.Org, NCBI)
Mesencephalic astrocyte-derived neurotrophic factor
Arginine-Rich Protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; MANF; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET MANF ARMET, ARP mesencephalic astrocyte derived neurotrophic factor mesencephalic astrocyte-derived neurotrophic factor|arginine-rich, mutated in early stage tumors*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MANF, check out the MANF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MANF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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