MANF Antibodies

Mesencephalic astrocyte-derived neurotrophic factor (MANF)

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (PubMed:12794311). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity).

Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (PubMed:18561914, PubMed:22637475, PubMed:29497057).

Gene Information

Human
Gene Name:	MANF
Uniprot:	P55145
Entrez:	7873

Family

Belongs to:
ARMET family

Alternative Names

Arginine-Rich Protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; MANF; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET

Molecular Weight

Mass (kDA):
20.7 kDA

Genomic Context

Human
Location:	3p21.2
Sequence:	3; NC_000003.12 (51385291..51389397)

Subcellular Localizations

Secreted. Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Retained in the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475, PubMed:29497057).

Diseases

• Parkinson Disease
• Neoplasms
• Malignant Neoplasms
• Ischemia
• Endoplasmic Reticulum Stress
• Neurodegenerative Disorders
• Nervousness
• Brain Ischemia
• Physiological Stress
• Infarction
• Carcinoma
• Renal Cell Carcinoma
• Myocardial Ischemia

• Secondary Parkinson Disease
• Cerebral Ischemia
• Kidney Neoplasm
• Brain Injuries
• Non-small Cell Lung Carcinoma
• Arterial Occlusion
• Hypertrophy

Pathways

• Localization
• Cell Death
• Transport
• Secretion
• Fertilization
• Nuclear Import
• Protein Folding
• Constitutive Secretory Pathway
• Brain Development
• Complement Activation
• Neurogenesis
• Nuclear Transport
• Cytokinesis

• Mrna Splicing
• Translation
• Cognition
• Exocytosis
• Blood Coagulation
• Secretory Pathway
• Cell Proliferation

PTMs

• Glycosylation

• Biotinylation

Research Areas

• Cancer
• DNA Repair

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MANF

Best Uses of the MANF Marker

You may have heard the term MANF Marker. But do you know what it is and what its best uses are? We will be discussing Anti -SLCO4A1 and MANF in this article. You might be interested in learning how these antibodies work in different cell types and how you can apply them in your research. This article will also address the importance of MANF Marker in cell biological research.

MANF Marker

There are many types MANF markers. The marking substrate can be either a liquid paint or a crayon, pencil, lead, or another material. The container can be made from plastic, metal or even paper wrap. The tip may be made of synthetic fibers, felts, or polyester. A metal ball-bearing could also be used to mark surfaces. The formula of the marking substance will depend on the type of surface to be marked. There are also markers made for general purpose use.

MANF Markers are a great tool for many writing tasks. These high-quality markers work well for a variety purposes, including office and home. These markers are great for entertaining children or making an impression at work. MANF markers provide vibrant colors and excellent quality. Browse through the online stores to find the right manf marker for you. Once you have found the right one, you can order it from the manufacturer.

Permanent markers are great for labeling projects due to their ability to resist fading. You can personalize your coloring experience with many different types. There are many sizes of MANF markers, including fine and broad tips. Some markers are available with different inks, so you can use them on different surfaces. Take a look at our list to find the best permanent marker for MANF.

Anti-CD8A antibody

The human CD8a antibody reacts with the alpha subunit of mouse CD8, which is composed of two chains: the CD8a and CD8b. CD8a belongs to the T-cell receptor, which binds antigen bearing MHC class I molecules. Several T-cells, including NK cells as well as lymphoid dendritic cell populations, also have the CD8asubunit.

To build a library, we use human peripheral blood mononuclear (HPMC) cells. The genes of the variable heavy (VH) and light (VL) chain-regions are amplified by reverse transcription. The resulting cDNA sequencing are then ligated into phages. The phage display technology is used to identify high-affinity antibodies in antibody gene mutant libraries. This technique employs phage display technology with stringent biopanning conditions.

The global market for therapeutic antibodies is expanding quickly. These antibodies have become the most widely prescribed drugs in the last five years. The antibody drugs have shown fewer side effects than those produced by conventional methods. Antibodies have been the most popular new drug class in the pharmaceutical sector. In fact, the top ten bestselling drugs worldwide in 2018 were all biologics.

The MANF markers are most commonly used for identifying antibodies. The MANF marker has a role in the immune response to bacterial infections. For example, anti-CD8A antibody could be used to detect the presence microbial infections within a mouse. Researchers have discovered that the MANF marker can identify a number of bacterial species that can cause infectious diseases.

Anti-SLCO4A1 antibody

The Anti-SLCO4A1 antibody is available from Boster Bio. It reacts with Human, Mouse and Rat. You can also use the antibody for cell culture experiments. It has been validated across a variety of applications, including Western Blotting, Immunohistochemistry, and ELISA. These antibodies are essential for researchers who want to conduct experiments that meet the highest standards. So, if you are looking for a high-affinity antibody that targets a particular protein, you should look no further.

The company has extensive experience in the development and production of high-affinity antibody. Their antibodies have been successfully validated on multiple platforms. These properties make them a top choice for research laboratories. However, before choosing this antibody, ensure that you understand the limitations of the product. It may not be suitable for all applications. Always check with your research team before you use it. There are several other options that you can consider, however.

SLCO4A1 is a member of the family of solute carriers that transport molecules into cells. Patients with laryngeal carcinoma have been shown to have high levels of the antigen SLC4A1A1-AS1. The antibody inhibited tumor growth, cell death, proliferation and cell death in mice. Subcellular fractionation assays confirmed the protein's expression in cells and its distribution. In addition, luciferase reporter assays confirmed its interaction with other molecules.

Anti-SLC10A1 antibody

Boster Bio's Anti-SLC10A1 antibody is highly specific and sensitive. It is intended for detection of SLC10A1 within various cells. This antibody is synthesized using synthetic peptides. It comes from the middle of the human SLC10A1 proteins. Post-translational modifications, relative charges and other factors may lead to a different molecularweight. The antibody must be freed from glycerol accumulated at -20C during storage before being used in downstream applications.

This monoclonal antibody is made from a synthetic sequence that is similar to the human SLC10A1 N-terminal sequence. It differs slightly from the sequences of mouse and rat. Anti-SLC10A1 antibodies can be stored in the freezer at temperatures between -20 and 4 degrees Celsius. However, if you freeze these antibodies frequently, they might not function properly.

ELISA Kits

ELISA kits for the MANF gene marker are available in several formats. They are used in a variety applications, including screening and monitoring for toxins. Sensitive ELISAs, for example, can detect the effects hormones and steroids on the MANF genetic gene. They are also highly sensitive for certain components of the Wnt signaling pathway, including Dkk-1 and b-catenin.

ELISA involves the use of antibodies to detect the presence of a specific antigen. The detection antibody is bound to an antigen in this method. The antigen and detection antibodies form a sandwich. The antigen and the detection antibody are then placed onto the microplate, which is then rinsed to remove unbound enzyme conjugates. This ensures that the measurement result remains objective.

Trinity Biotech's commercial ELISA kits for the MANF marker were deemed most suitable for epidemiological research. It helped describe the transmission of malaria in two pre-elimination settings. These kits can detect all four species of human malaria, despite their inaccuracy. These kits have not been tested in DBS samples. Further research is needed to validate their performance in real world applications.

Picoband ELISA kits

All the reagents, instructions, and equipment required to conduct an experiment on a plate are included in ELISA kits. The test detects molecules, then quantifies them by measuring antibody binding to the target. There are many formats of ELISA. Each variant has its own antigen interaction principle. Picokine(tm) ELISA kits use high affinity antibodies to detect protein in its native form. The company also validates ELISA kits against relevant superfamilies, immunogenic proteins.

ELISA kits that include Picoband and/or MANF markers from Diaclone can be used to measure the quantitative or qualitative levels of these proteins. These kits come with EASYview coloured dye reagents that make pipetting easier. These reagents are compatible with many other ELISA kits. They also provide an added visual aid to aid in filling Diaclone ELISA plates.

Picoband and/or MANF markers can be used in ELISA kits. They are available in many formats. Sandwich ELISA kits are the most popular, followed by uncoated ELISA kit. Each of these kits contains the reagents needed for sandwich ELISA development. Picoband antibodies and the MANF marker are paired and conjugated together with an ELISA substrate.

ELISA is a sensitive method that can detect the presence of a variety of cancers. The results from a single sample will prove to be accurate. MANF is a very popular cancer drug. However, the method is not straightforward. Picoband ELISA kits and MANF markers have many benefits. They are easy to use, reliable and reproducible. They can be purchased from any ELISA supplier.