Human MCP-1 / CCL2 ELISA Kit PicoKine®

CCL2 ELISA kit for Human

Human MCP-1 / CCL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL2 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0441
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

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Product Name

Human MCP-1 / CCL2 ELISA Kit PicoKine®

View all CCL2 ELISA kits

SKU/Catalog Number

EK0441

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MCP-1 / CCL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL2 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human MCP-1 / CCL2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0441)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-T99

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross-reactivity

There is cross-reactivity with human Eotaxin, MCP-3<1%.

Reactive Species

EK0441 is reactive to CCL2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK0441 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL2

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Kit Components

Catalog Number Description Quantity
EK0441-CAP Anti-Human CCL2 Pre-coated 96-well strip microplate 1
EK0441-ST Human CCL2 Standard 2 vials, 10 ng/tube
EK0441-DA Human CCL2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0250.0710.1540.2440.4661.0521.2981.892

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CCL2, Dilution ratio of 1:1, concentration in serum is around 500pg/ml.

Some articles we found to cite concentrations of CCL2 in samples: 23629827 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MCP-1 / CCL2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3115947730148481
Standard deviation1.558.7432.911.989.7637.51
CV (%)5%5.5%6.9%6.6%6.6%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131333735342.236.5%
Sample 21591391561581538.155.3%
Sample 347742142345644423.465.2%
*number of samples for each test n=16.

Gene/Protein Information For CCL2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P13500

Gene ID

6347

Gene Name

CCL2

Full Name

C-C motif chemokine 2

Weight

11.025kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF CCL2 GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF C-C motif chemokine ligand 2 C-C motif chemokine 2|chemokine (C-C motif) ligand 2|monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte chemotactic protein 1|monocyte secretory protein JE|small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|small inducible cytokine subfamily A (Cys-Cys), member 2|small-inducible cytokine A2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCL2, check out the CCL2 Infographic

CCL2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0441 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Identification of genes involved in enterovirus 71 infected SK-N-SH cells

Vaccarin prevents ox-LDL-induced HUVEC EndMT, inflammation and apoptosis by suppressing ROS/p38 MAPK signaling

Comparative analysis of MCP-1 and TF in elderly patients with acute exacerbations of COPD and its clinical significance.

CCL2-SQSTM1 positive feedback loop suppresses autophagy to promote chemoresistance in gastric cancer

Endogenous PGE2 induces MCP‑1 expression via EP4/p38 MAPK signaling in melanoma

A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-α activity

Thalidomide decreases high glucose‑induced extracellular matrix protein synthesis in mesangial cells via the AMPK pathway

Anti-Inflammatory Effect of the Blueberry Anthocyanins Malvidin-3-Glucoside and Malvidin-3-Galactoside in Endothelial Cells

Downregulation of S1P Lyase Improves Barrier Function in Human Cerebral Microvascular Endothelial Cells Following an Inflammatory Challenge

Downregulation of the S1P Transporter Spinster Homology Protein 2 (Spns2) Exerts an Anti-Fibrotic and Anti-Inflammatory Effect in Human Renal Proximal Tubular Epithelial Cells

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8 Customer Q&As for Human MCP-1 / CCL2 ELISA Kit PicoKine®

Question

Q: which procedure should I follow in order to thaw whole blood sample for CCL2 ELISA after freezing?

C. Mitchell

Verified customer

Asked: 2020-09-20

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-09-20

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-03-11

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

L. Lopez

Verified customer

Asked: 2019-04-02

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-04-02

Question

Q: What is the optimal O.D. value for CCL2 ELISA kit? I used your CCL2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CCL2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-05-15

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL2 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of CCL2 that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2018-05-15

Question

Q: how to proceed with the analysis of ELISA data? I measured CCL2 level in plasma.

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-04-19

Question

Q: can I use citrate plasma as samples in Human CCL2 Picokine® ELISA Kit (Catalog # EK0441)?

Verified Customer

Verified customer

Asked: 2018-04-02

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CCL2 causing disruption of its protein structure. CCL2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CCL2 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-04-02

Question

Q: if the enzyme conjugated CCL2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL2 antigen?

Verified Customer

Verified customer

Asked: 2017-01-30

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-01-30

Question

What serum dilution should be used for this antibody EK0441? I will be using human serum samples.

Verified Customer

Verified customer

Asked: 2013-09-23

Answer

We recommend to assay the human serum samples neat with standard buffer. The serum must be 2 fold dilution.

Boster Scientific Support

Answered: 2013-09-23

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