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Product Info Summary
| SKU: | EK1201 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. |
Product info
Product Name
Human MFGE8/Lactadherin ELISA Kit PicoKine®
SKU/Catalog Number
EK1201
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MFGE8/Lactadherin ELISA Kit PicoKine™ (96 Tests). Quantitate Human MFGE8 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MFGE8/Lactadherin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1201)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L24-C387
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1201 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1201 is reactive to MFGE8 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EK1201 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MFG-E8
MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1201-CAP | Anti-Human MFGE8 Pre-coated 96-well strip microplate | 1 |
| EK1201-ST | Human MFGE8 Standard | 2 vials, 10 ng/tube |
| EK1201-DA | Human MFGE8 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.091 | 0.150 | 0.176 | 0.260 | 0.443 | 0.738 | 1.276 | 1.846 |
Data Example Images
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Human MFGE8/Lactadherin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MFG-E8, Dilution ratio of 1:1, concentration in serum is around 800 pg/ml..
Some articles we found to cite concentrations of MFG-E8 in samples: 24561551 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MFGE8/Lactadherin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 100 | 660 | 1196 | 101 | 717 | 1149 |
| Standard deviation | 5.8 | 36.96 | 90.89 | 8.28 | 41.58 | 102.26 |
| CV (%) | 5.8% | 5.6% | 7.6% | 8.2% | 5.8% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MFG-E8 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 100 | 98 | 103 | 96 | 99 | 2.58 | 2.6% |
| Sample 2 | 660 | 663 | 784 | 706 | 703 | 50.04 | 7.1% |
| Sample 3 | 1196 | 1025 | 1141 | 1197 | 1139 | 70.01 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For MFGE8 (Source: Uniprot.Org, NCBI)
Gene Name
MFGE8
Full Name
Lactadherin
Weight
43.105kDa
Alternative Names
BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47 MFGE8 BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47 milk fat globule EGF and factor V/VIII domain containing lactadherin|O-acetyl disialoganglioside synthase|breast epithelial antigen BA46|medin|milk fat globule-EGF factor 8 protein|sperm associated antigen 10|sperm surface protein hP47
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MFGE8, check out the MFGE8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MFGE8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MFGE8/Lactadherin ELISA Kit PicoKine® (EK1201)
Hello CJ!
EK1201 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A rare missense variant in the milk fat globule-EGF factor 8 (MFGE8) increases T2DM susceptibility and cardiovascular disease risk with population-specific effects
A rare missense variant in the milk fat globule-EGF factor 8 (MFGE8) increases T2DM susceptibility and cardiovascular disease risk with population-specific effects
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human MFGE8/Lactadherin ELISA Kit PicoKine®
Question
Can EK1201 be used for measuring MFGE8 from extracted exosomes?
Verified customer
Asked: 2022-04-14
Answer
Our lab hasn't detected extracted exosomes with Human MFGE8/Lactadherin ELISA Kit PicoKine® (EK1201). We are uncertain if the kit can be used. We suggest to run a pilot test with the kit to see if it would work for the specific samples.
Boster Scientific Support
Answered: 2022-04-15
Question
How much neat serum/plasma would be added to each well on sample dilutions using EK1201?
Verified customer
Asked: 2022-04-07
Answer
For Human MFGE8/Lactadherin ELISA Kit PicoKine® (EK1201), the amount of neat serum/plasma to be added is 10-20ul. The dilution ratio should be 5-10 times.
Boster Scientific Support
Answered: 2022-04-07
Question
Can EK1201 be used for measuring MFGE8 from extracted exosomes?
Verified customer
Asked: 2022-03-14
Answer
Our lab hasn't detected extracted exosomes with Human MFGE8/Lactadherin ELISA Kit PicoKine® (EK1201). We are uncertain if the kit can be used. We suggest to run a pilot test with the kit to see if it would work for the specific samples.
Boster Scientific Support
Answered: 2022-03-15
Question
Q: What is the optimal O.D. value for MFG-E8 ELISA kit? I performed your MFG-E8 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain MFG-E8 even though the O.D. values are not very high?
C. Taylor
Verified customer
Asked: 2020-08-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MFG-E8 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MFG-E8 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-08-15
Question
Q: which procedure should I follow in order to thaw whole blood sample for MFG-E8 ELISA after freezing?
B. Zhou
Verified customer
Asked: 2019-05-15
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MFG-E8 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-05-15
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-05-15
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-01-21
Question
Q: is it okay to use citrate plasma as samples in Human MFG-E8 Picokine® ELISA Kit (Catalog # EK1201)?
Verified Customer
Verified customer
Asked: 2018-07-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of MFG-E8 causing disruption of its protein structure. MFG-E8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the MFG-E8 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-07-21
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis MFG-E8 using the EK1201 Human MFG-E8 Picokine® ELISA Kit?
S. Wright
Verified customer
Asked: 2018-05-10
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-05-10
