c-PLA2 Colorimetric Cell-Based ELISA Kit

The c-PLA2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor c-PLA2 protein expression profile in cells. The kit can be used for measuring the relative amounts of c-PLA2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on c-PLA2.

Product Info Summary

SKU: EKC1138
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

c-PLA2 Colorimetric Cell-Based ELISA Kit

See all PLA2G4A products

SKU/Catalog Number

EKC1138

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The c-PLA2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor c-PLA2 protein expression profile in cells. The kit can be used for measuring the relative amounts of c-PLA2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on c-PLA2.

Storage & Handling

4°C/6 Months

Cite This Product

c-PLA2 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1138)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1138 is reactive to PLA2G4A in Human, Mouse, Rat samples

Application Guarantee

EKC1138 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-PLA2G4A Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For PLA2G4A (Source: Uniprot.Org, NCBI)

Uniprot ID

P47712

Gene ID

5321

Gene Name

PLA2G4A

Full Name

Cytosolic phospholipase A2

Weight

85.239kDa

Alternative Names

calcium-dependent phospholipid-binding protein; cPLA2; cPLA2-alpha; lysophospholipase; MGC126350; phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent); PLA2G4A; PLA2G4cytosolic phospholipase A2 PLA2G4A GURDP, PLA2G4, cPLA2, cPLA2-alpha phospholipase A2 group IVA cytosolic phospholipase A2|calcium-dependent phospholipid-binding protein|lysophospholipase|phosphatidylcholine 2-acylhydrolase|phospholipase A2, group IVA (cytosolic, calcium-dependent)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PLA2G4A, check out the PLA2G4A Infographic

PLA2G4A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PLA2G4A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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