Anti-Phospholipase A2/PLA2G4A Antibody

Boster Bio Anti-Phospholipase A2/PLA2G4A Antibody catalog # PA2047. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2047
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Phospholipase A2/PLA2G4A Antibody

See all PLA2G4A products

SKU/Catalog Number

PA2047

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Phospholipase A2/PLA2G4A Antibody catalog # PA2047. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospholipase A2/PLA2G4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2047)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PLA2G4A (686-700aa PNQAFKRLHDLMHFN), different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2047 is reactive to PLA2G4A in Human, Mouse, Rat

Applications

PA2047 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For PLA2G4A (Source: Uniprot.Org, NCBI)

Uniprot ID

P47712

Gene ID

5321

Gene Name

PLA2G4A

Full Name

Cytosolic phospholipase A2

Weight

85.239kDa

Alternative Names

calcium-dependent phospholipid-binding protein; cPLA2; cPLA2-alpha; lysophospholipase; MGC126350; phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent); PLA2G4A; PLA2G4cytosolic phospholipase A2 PLA2G4A GURDP, PLA2G4, cPLA2, cPLA2-alpha phospholipase A2 group IVA cytosolic phospholipase A2|calcium-dependent phospholipid-binding protein|lysophospholipase|phosphatidylcholine 2-acylhydrolase|phospholipase A2, group IVA (cytosolic, calcium-dependent)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PLA2G4A, check out the PLA2G4A Infographic

PLA2G4A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PLA2G4A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-Phospholipase A2/PLA2G4A Antibody

Question

I see that the anti-Phospholipase A2/PLA2G4A antibody PA2047 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-07

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Phospholipase A2/PLA2G4A antibody PA2047. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-15

Question

I was wanting to use your anti-Phospholipase A2/PLA2G4A antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

As indicated on the product datasheet, PA2047 anti-Phospholipase A2/PLA2G4A antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-11

Question

Will anti-Phospholipase A2/PLA2G4A antibody PA2047 work for WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-01-25

Answer

According to the expression profile of cervix carcinoma, PLA2G4A is highly expressed in cervix carcinoma. So, it is likely that anti-Phospholipase A2/PLA2G4A antibody PA2047 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2019-01-25

Question

My question regarding product PA2047, anti-Phospholipase A2/PLA2G4A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2047 anti-Phospholipase A2/PLA2G4A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-18

Question

Is this PA2047 anti-Phospholipase A2/PLA2G4A antibody reactive to the isotypes of PLA2G4A?

Verified Customer

Verified customer

Asked: 2017-08-30

Answer

The immunogen of PA2047 anti-Phospholipase A2/PLA2G4A antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human PLA2G4A (686-700aa PNQAFKRLHDLMHFN), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-08-30

Size

Total: $240

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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PA2047
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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