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Product Info Summary
| SKU: | FEK0459 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma(heparin). |
| Assay Time: | 90 Minutes |
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Human MMP-2 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0459
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MMP-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate human MMP-2 in cell culture supernatants, serum and plasma(heparin). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human MMP-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0459)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: G311-S426
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0459 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0459 is reactive to INHBA in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma(heparin).
Application Guarantee
FEK0459 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of INHBA
Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0459-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0459-ST | Human INHBA Standard | 2 | 40 ng/tube |
| FEK0459-A | Human INHBA Antibody A | 1 | 3ml |
| FEK0459-B | Human INHBA Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 | 40,000 |
| O.D. | 0.015 | 0.190 | 0.335 | 0.627 | 1.138 | 1.738 | 1.905 | 2.079 |
Data Example Images
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Human MMP-2 PicoKine Quick ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-2 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 952 | 1335 | 18263 | 716 | 1356 | 10353 |
| Standard deviation | 53.03 | 97.05 | 1313.11 | 18.83 | 58.99 | 792 |
| CV(%) | 5.6% | 7.3% | 7.2% | 2.6% | 4.4% | 7.7% |
Protein Target Info & Infographic
Gene/Protein Information For INHBA (Source: Uniprot.Org, NCBI)
Gene Name
INHBA
Full Name
Weight
57.5 kDa
For more info on INHBA, check out the INHBA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for INHBA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
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