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Product Info Summary
| SKU: | M00561 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TIMP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00561
BM4980 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TIMP1 Rabbit Monoclonal Antibody catalog # M00561. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIMP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00561)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAOD-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TIMP1
Reactive Species
M00561 is reactive to TIMP1 in Human
Observed Molecular Weight
26 kDa
Calculated molecular weight
23.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00561 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human prostate cancer
Validation Images & Assay Conditions
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Western blot analysis of TIMP1 expression in human prostate cancer lysate.
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Knockdown of LY6K inhibits migration and invasion of CCSCs in vitro. Optical micrographs and the number of migrated ( A ) and invaded ( B ) CCSCs following siLY6K or siNC transfection for 48 h according to Transwell migration and invasion assay. n = 3. ( C ) Western blot analyses showing the protein levels of MMP-2, MMP-9, and TIMP-1 in CCSCs after treatment with siLY6K or siNC for 48 h. Values are the mean ± SE (n = 3). ** p < 0.01, *** p < 0.001, or **** p < 0.0001 indicates significant differences from the Mock and siNC group as assessed by one-way ANOVA with Tukey–Kramer multiple comparisons tests. LY6K, lymphocyte antigen 6, locus K; CCSC, colon cancer stem cells; MMP, matrix metalloproteinase; TIMP-1, tissue inhibitor of MMP-1; n.s., not significant.
Index in PubMed under a CC BY license. PMID: 39727968
Specific Publications For Anti-TIMP1 Rabbit Monoclonal Antibody (M00561)
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