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Product Info Summary
| SKU: | EK0814 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine®
SKU/Catalog Number
EK0814
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine™ (96 Tests). Quantitate Human MST1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0814)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q19-G711
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0814 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0814 is reactive to MST1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0814 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MSP/MST1
Macrophage Stimulating 1 (SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0814-CAP | Anti-Human MST1 Pre-coated 96-well strip microplate | 1 |
| EK0814-ST | Human MST1 Standard | 2 vials, 10 ng/tube |
| EK0814-DA | Human MST1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.000 | 0.045 | 0.077 | 0.148 | 0.302 | 0.635 | 1.173 | 1.985 |
Data Example Images
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Human MSP/MST1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MSP/MST1, Dilution ratio of 1:100, concentration in serum is 107-328 ng/ml..
Some articles we found to cite concentrations of MSP/MST1 in samples: 7691976 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 250 | 1105 | 6318 | 245 | 1017 | 6055 |
| Standard deviation | 19.25 | 44.2 | 398.03 | 23.52 | 53.9 | 399.63 |
| CV (%) | 7.7% | 4% | 5.3%% | 9.6% | 5.3% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MSP/MST1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 250 | 301 | 271 | 291 | 278 | 19.56 | 7% |
| Sample 2 | 1105 | 1177 | 1061 | 1161 | 1126 | 46.07 | 4% |
| Sample 3 | 6318 | 6544 | 5798 | 6359 | 6254 | 277.1 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For MST1 (Source: Uniprot.Org, NCBI)
Gene Name
MST1
Full Name
Hepatocyte growth factor-like protein
Weight
80.32kDa
Superfamily
peptidase S1 family
Alternative Names
D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2 MST1 D3F15S2, DNF15S2, HGFL, MSP, NF15S2 macrophage stimulating 1 hepatocyte growth factor-like protein|hepatocyte growth factor-like protein homolog|macrophage stimulating 1 (hepatocyte growth factor-like)|macrophage-stimulating protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MST1, check out the MST1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MST1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine® (EK0814)
Hello CJ!
EK0814 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang J, Ren Y, Guo X, Cheng H, Ye Y, Qi J, Yang C, You H. Mol Med Rep. 2015 May;11(5):3585-92. Doi: 10.3892/Mmr.2015.3164. Epub 2015 Jan 8. Alterations In Enhancer Of Zeste Homolog 2, Matrix Metalloproteinase-2 And Tissue Inhibitor Of Metalloprote...
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Human MSP Picokine® ELISA Kit (Catalog # EK0814)?
G. Perez
Verified customer
Asked: 2020-12-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of MSP causing degradation of its protein structure. MSP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the MSP ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-12-09
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of MSP in Human cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
J. Roberts
Verified customer
Asked: 2020-07-30
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MSP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-07-30
Question
Q: how can I thaw whole blood sample for MSP ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MSP for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-04-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-10-18
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification MSP using the EK0814 Human MSP Picokine® ELISA Kit?
W. Bhatt
Verified customer
Asked: 2019-09-09
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-09-09
Question
Q: if the enzyme conjugated MSP antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MSP antigen?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-08-09
Question
Q: how do I analyze ELISA data? I measured MSP level in cell lysates.
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-06-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
A. Singh
Verified customer
Asked: 2019-02-04
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-02-04
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
L. Zhang
Verified customer
Asked: 2018-05-31
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-31
