Mouse MSP/MST1 ELISA Kit PicoKine™

Mouse MSP/MST1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mst1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1189
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse MSP/MST1 ELISA Kit PicoKine™

See all MSP/MST1 products

SKU/Catalog Number

EK1189

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse MSP/MST1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mst1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse MSP/MST1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1189)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q19-E716

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1189 is reactive to Mst1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1189 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1189-CAP Anti-Mouse Mst1 Pre-coated 96-well strip microplate 1
EK1189-ST Mouse Mst1 Standard 2 vials, 10 ng/tube
EK1189-DA Mouse Mst1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0470.0710.1050.1770.3130.5691.0591.881

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MSP/MST1, Dilution ratio of 1:500, concentration in serum is around 1000ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse MSP/MST1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2571513586027614565996
Standard deviation11.5662.03322.312.9761.15425.71
CV (%)4.5%4.1%5.5%4.7%4.2%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MSP/MST1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12572832612642669.983.7%
Sample 21513149912871336140898.97%
Sample 358605664584159395826100.491.7%
*number of samples for each test n=16.

Gene/Protein Information For Mst1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P26928

Gene ID

15235

Gene Name

Mst1

Full Name

Hepatocyte growth factor-like protein

Weight

80.619kDa

Superfamily

peptidase S1 family

Alternative Names

D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2 Mst1|D3F15S2, D3F15S2h, D9H3F15S, D9H3F15S2, DNF15S, DNF15S2h, Hgf, Hgfl|macrophage stimulating 1 (hepatocyte growth factor-like)|hepatocyte growth factor-like protein|MSP|macrophage stimulatory protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mst1, check out the Mst1 Infographic

Mst1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mst1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Mouse MSP/MST1 ELISA Kit PicoKine™

Question

Q: how to thaw whole blood sample for MSP ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MSP for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-01-13

Question

Q: how do I analyze ELISA data? I have obtained MSP level in serum.

Verified Customer

Verified customer

Asked: 2019-12-26

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-12-26

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

T. Chen

Verified customer

Asked: 2019-07-07

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-07-07

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification MSP using the EK1189 Mouse MSP Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-04-30

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-04-30

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

A. Liu

Verified customer

Asked: 2016-07-02

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-07-02

Question

Q: can I use citrate plasma as samples in Mouse MSP Picokine® ELISA Kit (Catalog # EK1189)?

V. Mitchell

Verified customer

Asked: 2014-09-29

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of MSP causing degradation of its protein structure. MSP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MSP ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2014-09-29

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