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Product Info Summary
| SKU: | EK7069 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | Serum and Plasma |
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Product info
Product Name
Human Myoglobin OneStep ELISA Kit
SKU/Catalog Number
EK7069
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Human Myoglobin OneStep ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Myoglobin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7069)
Clonality of Antibodies
See Datasheet for details
Sensitivity
25 ng/ml
Assay Range
25-1000 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7069 is reactive to MB in Human samples
Validated Sample Types
Serum and Plasma
Application Guarantee
EK7069 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| 1. Microwell coated with murine monoclonal anti-myoglobin. | 12x8x1 |
| 2. Reference Standard Set | 0.5 ml |
| 3. Sample Diluent | 25 ml |
| 4. Enzyme Conjugate Reagent | 22 ml |
| 5. TMB Reagent | 11 ml |
| 6. Stop Solution | 11 ml |
| 7. Wash Concentrate 20x: 1 Bottle | 25 ml |
Standard Concentrations and example data
| OD 450 nm | Conc. ng/mL | |
|---|---|---|
| Std 1 | 0.045 | 0 |
| Std 2 | 0.191 | 25 |
| Std 3 | 0.628 | 100 |
| Std 4 | 1.445 | 250 |
| Std 5 | 2.178 | 500 |
| Std 6 | 2.896 | 1000 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Data Example Images
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Boster Kit Box
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Myoglobin, ELISA Target remove.
Protein Target Info & Infographic
Gene/Protein Information For MB (Source: Uniprot.org, NCBI)
Gene Name
MB
Full Name
Myoglobin
Weight
17.184kDa
Superfamily
globin family
Alternative Names
MB; MGC13548; Myoglobin; PVALB MB PVALB myoglobin myoglobin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MB, check out the MB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human Myoglobin OneStep ELISA Kit (EK7069)
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Customer Reviews
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