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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Nesfatin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NUCB2 in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1138)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: V25-L106
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1138 is reactive to NUCB2 in Human samples
Validated Sample Types
cell culture supernatants and serum.
EK1138 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1138-CAP||Anti-Human NUCB2 Pre-coated 96-well strip microplate||1|
|EK1138-ST||Human NUCB2 Standard||2 vials, 2 ng/tube|
|EK1138-DA||Human NUCB2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Nesfatin-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Nesfatin-1/Nucleobindin-2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For NUCB2 (Source: Uniprot.Org, NCBI)
DNA-binding protein NEFA; NEFA; NEFAGastric cancer antigen Zg4; Nesfatin1; Nesfatin-1; NUCB2; nucleobindin 2; Nucleobindin-2; nucleobinding 2 NUCB2 HEL-S-109, NEFA nucleobindin 2 nucleobindin-2|DNA-binding protein NEFA|epididymis secretory protein Li 109|gastric cancer antigen Zg4|nesfatin 1|novel DNA binding/EF-hand/leucine zipper protein|nucleobinding 2|prepronesfatin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NUCB2, check out the NUCB2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NUCB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1138 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Relationship between Nesfatin-1 Levels and SYNTAX Score in Patients with Non-ST Segment Elevation Myocardial Infarction
Increased concentrations of serum nesfatin-1 levels in childhood with idiopathic chronic malnutrition
Algul S, Ozdenk C, Ozcelik O. Biol Sport. 2017 Dec;34(4):339-344. doi: 10.5114/biolsport.2017.69821. Epub 2017 Sep 20. Variations in leptin, nesfatin-1 and irisin levels induced by aerobic exercise in young trained and untrained male subjects
??atl?? G, An??k A, K??me T, ??alan ??g, D??ndar Bn, B??ber E, Abac?? A. J Endocrinol Invest. 2015 Aug;38(8):909-13. Doi: 10.1007/S40618-015-0277-8. Epub 2015 Apr 2. Serum Nesfatin-1 And Leptin Levels In Non-Obese Girls With Premature Thelarche.
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