Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine™

Human Nesfatin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NUCB2 in cell culture supernatants and serum. Sensitivity: 10pg/ml. Cited in 4 publication(s).

Product Info Summary

SKU: EK1138
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine™

See all Nesfatin-1/Nucleobindin-2 products

SKU/Catalog Number

EK1138

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Nesfatin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NUCB2 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1138)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V25-L106

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1138 is reactive to NUCB2 in Human samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK1138 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1138-CAP Anti-Human NUCB2 Pre-coated 96-well strip microplate 1
EK1138-ST Human NUCB2 Standard 2 vials, 2 ng/tube
EK1138-DA Human NUCB2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0320.0860.1060.1960.4320.7551.3702.094

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)58278993622861045
Standard deviation2.5517.5157.203.4120.5974.19
CV (%)4.4%7.2%5.6%5.5%7.2%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Nesfatin-1/Nucleobindin-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158504955533.676.9%
Sample 227824927225026212.934.9%
Sample 3993100491484693964.066.8%
*number of samples for each test n=16.

Gene/Protein Information For NUCB2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P80303

Gene ID

4925

Gene Name

NUCB2

Full Name

Nucleobindin-2

Weight

50.223kDa

Superfamily

nucleobindin family

Alternative Names

DNA-binding protein NEFA; NEFA; NEFAGastric cancer antigen Zg4; Nesfatin1; Nesfatin-1; NUCB2; nucleobindin 2; Nucleobindin-2; nucleobinding 2 NUCB2 HEL-S-109, NEFA nucleobindin 2 nucleobindin-2|DNA-binding protein NEFA|epididymis secretory protein Li 109|gastric cancer antigen Zg4|nesfatin 1|novel DNA binding/EF-hand/leucine zipper protein|nucleobinding 2|prepronesfatin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NUCB2, check out the NUCB2 Infographic

NUCB2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NUCB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1138 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The Relationship between Nesfatin-1 Levels and SYNTAX Score in Patients with Non-ST Segment Elevation Myocardial Infarction

Increased concentrations of serum nesfatin-1 levels in childhood with idiopathic chronic malnutrition

Algul S, Ozdenk C, Ozcelik O. Biol Sport. 2017 Dec;34(4):339-344. doi: 10.5114/biolsport.2017.69821. Epub 2017 Sep 20. Variations in leptin, nesfatin-1 and irisin levels induced by aerobic exercise in young trained and untrained male subjects

??atl?? G, An??k A, K??me T, ??alan ??g, D??ndar Bn, B??ber E, Abac?? A. J Endocrinol Invest. 2015 Aug;38(8):909-13. Doi: 10.1007/S40618-015-0277-8. Epub 2015 Apr 2. Serum Nesfatin-1 And Leptin Levels In Non-Obese Girls With Premature Thelarche.

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3 Customer Q&As for Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine™

Question

Q: Can Nesfatin-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Singh

Verified customer

Asked: 2020-07-04

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-07-04

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-07-17

Question

Q: how can I thaw whole blood sample for Nesfatin-1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Nesfatin-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-03-01

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