Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™

Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NRG1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1378
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™

See all NRG1 products

SKU/Catalog Number

EK1378

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human NRG1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1378)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-K246

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1378 is reactive to NRG1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1378 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1378-CAP Anti-Human NRG1 Pre-coated 96-well strip microplate 1
EK1378-ST Human NRG1 Standard 2 vials, 10 ng/tube
EK1378-DA Human NRG1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0800.1260.1710.1840.3120.5211.0181.894

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect NRG1, Dilution ratio of 1:10, concentration in serum is 2-40ng/ml.

Some articles we found to cite concentrations of NRG1 in samples: 22599357 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)15862119001596071910
Standard deviation12.1630.4281.714.4640.0695.5
CV (%)7.7%4.9%4.3%9.1%6.6%5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of NRG1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11581501511351488.385.6%
Sample 262167860171165243.926.7%
Sample 31900186620821930194482.554.2%
*number of samples for each test n=16.

Gene/Protein Information For NRG1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q02297

Gene ID

3084

Gene Name

NRG1

Full Name

Pro-neuregulin-1, membrane-bound isoform

Weight

70.392kDa

Superfamily

neuregulin family

Alternative Names

ARIA; GGF2; GGFglial growth factor; Heregulin-1; HGL; HGLneu differentiation factor; HRG; HRG1; HRG1-alpha; HRG1-beta 1; HRGAneuregulin 1 type IV beta 1a; MST131; MSTP131; NDFheregulin, alpha (45kD, ERBB2 p185-activator); neuregulin 1 type IV beta 3; neuregulin 1; Neuregulin1; Neuregulin-1; NRG1; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF NRG1 ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF-IT2, SMDF, NRG1 neuregulin 1 pro-neuregulin-1, membrane-bound isoform|acetylcholine receptor-inducing activity|glial growth factor 2|heregulin, alpha (45kD, ERBB2 p185-activator)|neu differentiation factor|pro-NRG1|sensory and motor neuron derived factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NRG1, check out the NRG1 Infographic

NRG1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NRG1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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11 Customer Q&As for Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Human NRG1 Picokine® ELISA Kit (Catalog # EK1378)?

Verified Customer

Verified customer

Asked: 2020-11-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of NRG1 causing disruption of its protein structure. NRG1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the NRG1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-11-22

Question

Will EK1378 cross-react with mouse NRG1-beta 1?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

Our lab technicians think it would most likely cross reacts with mouse NRG1-beta 1.

Boster Scientific Support

Answered: 2020-02-03

Question

Do you have a recommended number or density of cells to prepare for EK1378 assay in Cell culure supernants and Cell lysates?

Verified customer

Asked: 2019-05-12

Answer

For the Human Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine™ (EK1378), the number of cells we recommened for cell culure supernants and cell lysates is 106 cells/mL.

Boster Scientific Support

Answered: 2019-05-12

Question

Q: how to thaw whole blood sample for NRG1 ELISA after freezing?

T. Collins

Verified customer

Asked: 2019-04-26

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test NRG1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-04-26

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

M. Nelson

Verified customer

Asked: 2019-01-09

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-01-09

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-10-17

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-10-17

Question

Q: What is the optimal O.D. value for NRG1 ELISA kit? I used your NRG1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain NRG1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-09-30

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this NRG1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of NRG1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-09-30

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-08-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-08-25

Question

Will EK1378 detects all neuregulin 1 isoforms or just the beta isoform?

Verified Customer

Verified customer

Asked: 2017-07-19

Answer

EK1378 detects just the beta isoform.

Boster Scientific Support

Answered: 2017-07-19

Question

Q: if the enzyme conjugated NRG1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the NRG1 antigen?

Verified Customer

Verified customer

Asked: 2016-04-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-04-19

Question

A customer is wondering if EK1378 detects all neuregulin 1 isoforms or just the beta isoform as per the title?

Verified Customer

Verified customer

Asked: 2013-06-18

Answer

EK1378 detects just the beta isoform.

Boster Scientific Support

Answered: 2013-06-18

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