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Product Info Summary
| SKU: | EK1628 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human NT-proBNP/NPPB ELISA Kit PicoKine®
SKU/Catalog Number
EK1628
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human NT-proBNP/NPPB ELISA Kit PicoKine® (96 Tests). Quantitate Human NT-proBNP/NPPB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human NT-proBNP/NPPB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1628)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: H27-H134
Sensitivity
<20 pg/ml
Assay Range
156pg/ml-10000pg/ml
Standard Dilution Instructions
See datasheet of EK1628 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1628 is reactive to NPPB in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK1628 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of NPPB
NPPB is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1628-CAP | Anti-Human NPPB Pre-coated 96-well strip microplate | 1 |
| EK1628-ST | Human NPPB Standard | 2 vials, 10 ng/tube |
| EK1628-DA | Human NPPB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.034 | 0.074 | 0.1211 | 0.207 | 0.341 | 0.644 | 1.198 | 2.485 |
Data Example Images
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Human NT-proBNP/NPPB ELISA Kit PicoKine standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human NT-proBNP/NPPB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 165 | 827 | 2641 | 181 | 779 | 2720 |
| Standard deviation | 3.61 | 41.18 | 200.98 | 8.87 | 59.83 | 110.70 |
| CV (%) | 2.2% | 5.0% | 7.6% | 4.9% | 7.7% | 4.1% |
Specific Publications For Human NT-proBNP/NPPB ELISA Kit PicoKine® (EK1628)
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