Human NRCAM ELISA Kit PicoKine®

NRCAM ELISA kit for Human

Human NRCAM ELISA Kit PicoKine™ (96 Tests). Quantitate Human NRCAM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1408
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human NRCAM ELISA Kit PicoKine®

View all NRCAM ELISA kits

SKU/Catalog Number

EK1408

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human NRCAM ELISA Kit PicoKine™ (96 Tests). Quantitate Human NRCAM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human NRCAM ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1408)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L30-N600

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1408 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1408 is reactive to NRCAM in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1408 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of NRCAM

Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.

Kit Components

Catalog Number Description Quantity
EK1408-CAP Anti-Human NRCAM Pre-coated 96-well strip microplate 1
EK1408-ST Human NRCAM Standard 2 vials, 10 ng/tube
EK1408-DA Human NRCAM Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0580.1320.210.3380.6041.0271.5312.037

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human NRCAM ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12281418671158891724
Standard deviation5.4935102.685.7554.22125.85
CV (%)4.5%4.3%5.5%5%6.1%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of NRCAM in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11221111141281186.685.6%
Sample 281476174276977126.423.4%
Sample 318672040172120871928145.257.5%
*number of samples for each test n=16.

Gene/Protein Information For NRCAM (Source: Uniprot.Org, NCBI)

Gene Name

NRCAM

Full Name

Neuronal cell adhesion molecule

Weight

143.89kDa

Superfamily

immunoglobulin superfamily

Alternative Names

Bravo; hBravo; KIAA0343Neuronal surface protein Bravo; MGC138845; MGC138846; neuronal cell adhesion molecule; NgCAM-related cell adhesion molecule; ng-CAM-related; NrCAM; nr-CAM NRCAM neuronal cell adhesion molecule neuronal cell adhesion molecule|NgCAM-related cell adhesion molecule|neuronal surface protein Bravo

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NRCAM, check out the NRCAM Infographic

NRCAM infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NRCAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EK1408

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human NRCAM ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human NRCAM ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

8 Customer Q&As for Human NRCAM ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

V. Zhang

Verified customer

Asked: 2020-10-16

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-10-16

Question

Q: Can NRCAM ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-08-29

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-08-29

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

P. Davis

Verified customer

Asked: 2019-06-28

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-06-28

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

J. Varadkar

Verified customer

Asked: 2019-01-11

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-01-11

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of NRCAM in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

S. Johnson

Verified customer

Asked: 2018-12-18

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the NRCAM ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-12-18

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

J. Wang

Verified customer

Asked: 2017-08-30

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-08-30

Question

Q: is it okay to use citrate plasma as samples in Human NRCAM Picokine® ELISA Kit (Catalog # EK1408)?

Verified Customer

Verified customer

Asked: 2016-12-27

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of NRCAM causing degradation of its protein structure. NRCAM may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the NRCAM ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-12-27

Question

Q: how do I analyze ELISA data? I measured NRCAM level in cell lysates.

Verified Customer

Verified customer

Asked: 2014-01-09

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2014-01-09

$499
In stock, 1 left.

Order within 73 hours and 28 minutes to receive by Tue Apr 2

Get A Quote
Eddy test
In stock
Order Product
EK1408
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.