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Product Info Summary
| SKU: | EK1528 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1528
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Peroxiredoxin 2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PRDX2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1528)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A2-N198
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1528 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1528 is reactive to PRDX2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1528 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PRDX2
Peroxiredoxin-2 is a protein that in humans is encoded by the PRDX2 gene. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8 (+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. It may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1528-CAP | Anti-Human PRDX2 Pre-coated 96-well strip microplate | 1 |
| EK1528-ST | Human PRDX2 Standard | 2 vials, 20 ng/tube |
| EK1528-DA | Human PRDX2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.028 | 0.08 | 0.141 | 0.247 | 0.456 | 0.872 | 1.641 | 2.585 |
Data Example Images
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Human Peroxiredoxin 2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PRDX2, Dilution ratio of 1:5, concentration in serum and plasma is around 13 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 405 | 3122 | 44534 | 445 | 3416 | 9285 |
| Standard deviation | 22.27 | 140.49 | 638.77 | 27.14 | 198.12 | 724.23 |
| CV (%) | 5.5% | 4.5% | 6.7% | 6.1% | 5.8% | 7.8% |
Specific Publications For Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine® (EK1528)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human PRDX2/Peroxiredoxin-2 ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for Peroxiredoxin 2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Peroxiredoxin 2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: if the enzyme conjugated Peroxiredoxin 2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Peroxiredoxin 2 antigen?
K. Turner
Verified customer
Asked: 2019-11-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-11-05
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Peroxiredoxin 2 using the EK1528 Human Peroxiredoxin 2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-10-26
Question
Q: how to proceed with the analysis of ELISA data? I have performed Peroxiredoxin 2 level in serum.
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2018-05-07
Question
Will EK1528 react with Prx1, Prx3, Prx4, Prx5 and Prx6 proteins?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
EK1528 will react with Prx1, Prx3, Prx4, Prx5 proteins.<br> Please see the following detected cross reactivity. <br> human recombinant Prx1, 0.2% <br>human recombinant Prx3, 5.1% <br>human recombinant Prx4, 19.8% <br>human recombinant Prx5, 0.3% <br>We do not have Prx6 protein for testing. It is uncertain if EK1528 will cross react with Prx6 protein.
Boster Scientific Support
Answered: 2017-08-08
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2014-04-28
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-04-28
Question
Q: What is the optimal O.D. value for Peroxiredoxin 2 ELISA kit? I used your Peroxiredoxin 2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Peroxiredoxin 2 even though the O.D. values are not very high?
A. Nelson
Verified customer
Asked: 2014-04-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Peroxiredoxin 2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Peroxiredoxin 2 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2014-04-19
