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Product Info Summary
| SKU: | EK2256 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human PILRB ELISA Kit PicoKine®
SKU/Catalog Number
EK2256
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PILRB ELISA Kit PicoKine® (96 Tests). Quantitate Human PILRB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 45 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PILRB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2256)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: HEK293; Immunogen sequence: Q20-A189
Sensitivity
<45 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2256 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2256 is reactive to PILRB in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2256 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PILR-beta
Paired immunoglobulin-like type 2 receptor beta is a protein that in humans is encoded by the PILRB gene. The paired immunoglobin-like type 2 receptors consist of highly related activating and inhibitory receptors that are involved in the regulation of many aspects of the immune system. The paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This gene encodes the activating member of the receptor pair and contains a truncated cytoplasmic tail relative to its inhibitory counterpart (PILRA), that has a long cytoplasmic tail with immunoreceptor tyrosine-based inhibitory (ITIM) motifs. This gene is thought to have arisen from a duplication of the inhibitory PILRA gene and evolved to acquire its activating function.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2256-CAP | Anti-Human PILRB Pre-coated 96-well strip microplate | 1 |
| EK2256-ST | Human PILRB Standard | 2 vials, 10 ng/tube |
| EK2256-DA | Human PILRB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.041 | 0.087 | 0.146 | 0.325 | 0.582 | 0.721 | 1.033 | 1.828 |
Data Example Images
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Human PRLRB PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PILRB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 116 | 321 | 2663 | 143 | 371 | 2601 |
| Standard deviation | 9.2 | 16.15 | 136.88 | 3.47 | 9.35 | 99.62 |
| CV(%) | 7.9% | 5% | 5.1% | 2.4% | 2.5% | 3.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PILR-beta in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 116 | 101 | 134 | 148 | 125 | 9.16 | 7.3% |
| Sample 2 | 321 | 303 | 263 | 351 | 310 | 19.07 | 6.2% |
| Sample 3 | 2663 | 1678 | 2544 | 1933 | 2205 | 141.31 | 6.4% |
Protein Target Info & Infographic
Gene/Protein Information For PILRB (Source: Uniprot.Org, NCBI)
Gene Name
PILRB
Full Name
Paired immunoglobulin-like type 2 receptor beta
Weight
25.542kDa
Alternative Names
activating receptor PILRbeta; Activating receptor PILR-beta; Cell surface receptor FDFACT; cell surface receptor FDFACT1; cell surface receptor FDFACT2; FDFACT; FDFACT1; FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobin-like type 2 receptor beta; paired immunoglobulin-like receptor beta; paired immunoglobulin-like type 2 receptor beta; PILRB; PILRbeta; PILR-beta PILRB FDFACT1, FDFACT2 paired immunoglobin like type 2 receptor beta paired immunoglobulin-like type 2 receptor beta|activating receptor PILR-beta|activating receptor PILRbeta|cell surface receptor FDFACT|cell surface receptor FDFACT1|cell surface receptor FDFACT2|paired immunoglobin-like receptor beta|paired immunoglobulin-like receptor beta
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PILRB, check out the PILRB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PILRB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PILRB ELISA Kit PicoKine® (EK2256)
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