Rat SCGB1A1/uteroglobin PicoKine® Quick ELISA Kit

Uteroglobin/SCGB1A1 ELISA kit, 90-minute one-step protocol

Rat SCGB1A1/uteroglobin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Scgb1a1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1636
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Assay Time: 90 Minutes

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Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Rat SCGB1A1/uteroglobin PicoKine® Quick ELISA Kit

See all Uteroglobin/SCGB1A1 products

SKU/Catalog Number

FEK1636

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat SCGB1A1/uteroglobin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Scgb1a1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat SCGB1A1/uteroglobin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1636)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S20-V96

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1636 is reactive to Scgb1a1 in Rat samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

FEK1636 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Uteroglobin/SCGB1A1

SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.

Kit Components

Catalog number Description Quantity
FEK1636-CAP 96-well plate precoated with anti-Rat Uteroglobin/SCGB1A1 antibody 1
FEK1636-ST lyophilized recombinant Rat Uteroglobin/SCGB1A1 standard 10 ng/tube
FEK1636-DA Biotinylated anti-Rat Uteroglobin/SCGB1A1 detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0420.0750.0990.1540.2630.5221.1352.156

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2201610449024116354849
Standard deviation8.8123.97298.6%410.36140.61374.3%2
CV (%)4%7.7%6.6%4.3%8.6%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Uteroglobin/SCGB1A1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 122025825925324716.036.4%
Sample 21610148313801552150685.635.6%
Sample 344904875448748494675186.973.9%
*number of samples for each test n=16.

Gene/Protein Information For Scgb1a1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17559

Gene ID

25575

Gene Name

Scgb1a1

Full Name

Uteroglobin

Weight

10.449kDa

Superfamily

secretoglobin family

Alternative Names

Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin Scgb1a1|CC10, CCSP, PCBB, Ugb|secretoglobin family 1A member 1|uteroglobin|CCPBP|PCB-binding protein|Uteroglobin (Clara cell secretory protein)|clara cell phospholipid-binding protein|clara cells 10 kDa secretory protein|secretoglobin, family 1A, member 1 (uteroglobin)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Scgb1a1, check out the Scgb1a1 Infographic

Scgb1a1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Scgb1a1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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