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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin or EDTA) and urine|
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Human PSG1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human PSG1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PSG1 in cell culture supernatants, serum, plasma (heparin or EDTA) and urine. Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PSG1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1997)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q35-P419
1.56 ng/ml - 100 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1997 is reactive to PSG1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin or EDTA) and urine
EK1997 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PSG1
The PSG1 is a semi-automatic sniper rifle， Pregnancy-specific beta-1 glycoprotein 1 (PSG-1) is also known as SP1, PSbG1, or B1G1, and is designated CD66f. It is mapped to 19q13.2. The human placenta is a multihormonal endocrine organ that produces hormones, enzymes, and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG, PSG) is a major product of the syncytiotrophoblast, reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women. PSG is a member of the immunoglobulin (Ig) superfamily.
|EK1997-CAP||Anti-Human PSG1 Pre-coated 96-well strip microplate||1|
|EK1997-ST||Human PSG1 Standard||2 vials, 100 ng/tube|
|EK1997-DA||Human PSG1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human PSG1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PSG1, Dilution ratio of 1:50, concentration in serum is around 1.25ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PSG1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of PSG1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PSG1 (Source: Uniprot.Org, NCBI)
Pregnancy-specific beta-1-glycoprotein 1
B1G1; B1G1CD66 antigen-like family member F; CD66f antigen; CD66f; DHFRP2; Fetal liver non-specific cross-reactive antigen 1/2; FLJ90598; FLJ90654; FL-NCA-1/2; PBG1; pregnancy specific beta-1-glycoprotein 1; pregnancy-specific B-1 glycoprotein; Pregnancy-specific beta-1 glycoprotein C/D; pregnancy-specific beta-1-glycoprotein 1; Pregnancy-specific glycoprotein 1; PS-beta-C/D; PS-beta-G-1; PSbG1; PSBG-1; PSBG1SP1; PSG1; PSG-1; PSG95; PSGGA; PSGGAPSG95; PSGIIA PSG1 B1G1, CD66f, DHFRP2, FL-NCA-1/2, PBG1, PS-beta-C/D, PS-beta-G-1, PSBG-1, PSBG1, PSG95, PSGGA, PSGIIA, SP1 pregnancy specific beta-1-glycoprotein 1 pregnancy-specific beta-1-glycoprotein 1|CD66 antigen-like family member F|fetal liver non-specific cross-reactive antigen 1/2|pregnancy-specific B-1 glycoprotein|pregnancy-specific beta-1 glycoprotein C/D*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PSG1, check out the PSG1 Infographic
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In this infographic you will see the following information for PSG1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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