Human PSP94 PicoKine® Quick ELISA Kit

PSP94/MSMB ELISA kit, 90-minute one-step protocol

Human PSP94 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human MSMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1207
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

Product Name

Human PSP94 PicoKine® Quick ELISA Kit

See all PSP94/MSMB products

SKU/Catalog Number

FEK1207

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PSP94 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human MSMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PSP94 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1207)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S21-I114

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1207 is reactive to MSMB in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK1207 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of PSP94/MSMB

Beta-microseminoprotein, also known as MSMB or PSP94, is a protein that in humans is encoded by the MSMB gene. It is a member of the immunoglobulin binding factor family. This gene is mapped to 10q11.23. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. The expression of the encoded protein is found to be decreased in prostate cancer. But transcripts of identical size were found in prostate, respiratory (tracheal, bronchial, and lung) tissues, and the mucosa of the gastric antrum. Thus, the protein may have functions other than those related to male reproduction. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues.

Kit Components

Catalog numberDescription QuantityVolume
FEK1207-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK1207-STHuman PSP94/MSMB Standard 2 10 ng/tube
FEK1207-CHuman MSMB Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.1070.2350.3030.5170.8871.4832.1422.385

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)16563315021435741323
Standard deviation12.8747.48112.658.7250.51127.01
CV (%)7.8%7.5%7.5%6.1%8.8%9.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of PSP94/MSMB in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11651551671521603.512.2%
Sample 263365066973667232.934.9%
Sample 31502124513971305136261.304.5%
*number of samples for each test n=16.

Gene/Protein Information For MSMB (Source: Uniprot.Org, NCBI)

Uniprot ID

P08118

Gene ID

4477

Gene Name

MSMB

Full Name

Beta-microseminoprotein

Weight

12.865kDa

Superfamily

beta-microseminoprotein family

Alternative Names

IGBF; MSMB; MSPB; PIP; PN44; PRPS; PSP57; PSP94 MSMB HPC13, IGBF, MSP, MSPB, PN44, PRPS, PSP, PSP-94, PSP57, PSP94 microseminoprotein beta beta-microseminoprotein|immunoglobulin binding factor|prostate secreted seminal plasma protein|prostate secretory protein of 94 amino acids|seminal plasma beta-inhibin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MSMB, check out the MSMB Infographic

MSMB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MSMB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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