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Product Info Summary
| SKU: | PROTP13500-5 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Human recombinant CCL2 (C-C motif chemokine ligand 2) protein, AF
View all CCL2 recombinant proteins
SKU/Catalog Number
PROTP13500-5
Size
5ug,20ug,100ug
Tag
His Tag (N-term)
Description
C-C Motif Chemokine Ligand 2 (CCL2) is an 8.44 kDa cytokine with 76 amino acid residues and is also known as monocyte chemoattractant protein 1 (MCP1). The CCL2 secreted from monocytes, macrophages, and dendritic cells. Upon binding with CCR4, it regulates many biological functions, such as recruitments of monocytes, memory T cells, and dendritic cells, response to TNF-α and Interferon-γ, angiogenesis, cellular homeostasis, and cytoskeleton organization.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant CCL2 (C-C motif chemokine ligand 2) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP13500-5)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
11.0 kDa
Molecular weight
The protein has a calculated MW of 9.49 kDa. The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to chemoattract BaF3 cells transfected with human CCR2A. The ED₅₀ for this effect is <20 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT with polyhistidine tag at the N-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human CCL2
Specific Publications For Human recombinant CCL2 (C-C motif chemokine ligand 2) protein, AF (PROTP13500-5)
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