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Product Info Summary
| SKU: | PROTP26583-2 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Human recombinant HMGB2 (High mobility group box 2) protein, AF
View all HMGB2 recombinant proteins
SKU/Catalog Number
PROTP26583-2
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
High mobility group protein B2, also known as HMGB2, is a member of the non-histone chromosomal high-mobility group protein family. High mobility group protein B2 is expressed as 25 kDa containing 209 amino acid residues. The function of High mobility group protein B2 is involved in transcription, chromatin remodeling and V(D)J recombination. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant HMGB2 (High mobility group box 2) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP26583-2)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
24.0 kDa
Molecular weight
The protein has a calculated MW of 24.84 kDa. The protein migrates as 34 kDa under reducing condition (SDS-PAGE analysis).
Activity
Testing in process
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPSAFFLFCSEHRPKIKSEHPGLSIGDTAKKLGEMWSEQSAKDKQPYEQKAAKLKEKYEKDIAAYRAKGKSEAGKKGPGRPTGSKKKNEPEDEEEEEEEEDEDEEEEDEDEE with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human HMGB2
Specific Publications For Human recombinant HMGB2 (High mobility group box 2) protein, AF (PROTP26583-2)
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