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Product Info Summary
| SKU: | PROTQ6EBC2-2 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Human recombinant IL-31 (Interleukin-31) protein, AF
View all IL31 recombinant proteins
SKU/Catalog Number
PROTQ6EBC2-2
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Interleukin 31 (IL-31) is an 18 kDa cytokine with 142 amino acid residues. IL-31 is mainly secreted from activated CD4+ T cells and binds to a receptor called IL-31RA. Upon binding to 31RA, IL-31 activates several signal pathways like MAPK, PI3K/AKT, and Jak/STAT pathways. It is critical in regulating many biological functions, such as cell proliferation, hematopoiesis, induction of cytokines, inflammation, and immune response.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant IL-31 (Interleukin-31) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ6EBC2-2)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
18.2 kDa
Molecular weight
The protein has a calculated MW of 16.78 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measured by its ability to induce A549 cells proliferation. The ED₅₀ for this effect is < 40 ng/mL
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MSHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human IL-31
Specific Publications For Human recombinant IL-31 (Interleukin-31) protein, AF (PROTQ6EBC2-2)
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