Human Resistin PicoKine® Quick ELISA Kit

Resistin ELISA kit, 120-minute protocol

Human Resistin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human RETN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: FEK0581
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Resistin PicoKine® Quick ELISA Kit

See all Resistin products

SKU/Catalog Number

FEK0581

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Resistin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human RETN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Resistin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0581)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: K19-P108

Sensitivity

<3 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of FEK0581 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0581 is reactive to RETN in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0581 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Resistin

Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

Kit Components

Catalog number Description Quantity
FEK0581-CAP 96-well plate precoated with anti-Human Resistin antibody 1
EK0581-ST lyophilized recombinant Human Resistin standard 10 ng/tube
FEK0581-DA Biotinylated anti-Human Resistin detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0130.1690.3060.4830.9261.3911.9202.305

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Resistin PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13563920671426362227
Standard deviation8.140.25161.228.5246.42177.83
CV (%)6%7.3%7.8%6%7.3%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Resistin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11351371431461404.433.1%
Sample 263965363473766541.726.2%
Sample 320672270233723372252110.674.9%
*number of samples for each test n=16.

Gene/Protein Information For RETN (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9HD89

Gene ID

56729

Gene Name

RETN

Full Name

Resistin

Weight

11419 MW

Superfamily

resistin/FIZZ family

Alternative Names

Resistin;Adipose tissue-specific secretory factor;ADSF;C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein;Cysteine-rich secreted protein A12-alpha-like 2;Cysteine-rich secreted protein FIZZ3;RETN;FIZZ3, HXCP1, RSTN;UNQ407/PRO1199; RETN ADSF, FIZZ31, RSTN, XCP1, RETN resistin resistin|C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1|adipose tissue-specific secretory factor|c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein|cysteine-rich secreted protein A12-alpha-like 2|cysteine-rich secreted protein FIZZ3|found in inflammatory zone 3|resistin delta2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RETN, check out the RETN Infographic

RETN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RETN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for FEK0581

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human Resistin PicoKine® Quick ELISA Kit?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Human Resistin PicoKine® Quick ELISA Kit

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Human Resistin PicoKine® Quick ELISA Kit

$499
Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
In stock
Order Product
FEK0581
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.