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Product Info Summary
| SKU: | EK1874 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) and urine. |
Product info
Product Name
Human Serpin A12 ELISA Kit PicoKine®
View all Serpin A12 ELISA kits
SKU/Catalog Number
EK1874
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Serpin A12 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINA12 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Serpin A12 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1874)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L21-K414
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1874 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1874 is reactive to Serpina12 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA) and urine.
Application Guarantee
EK1874 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin A12
This gene is mapped to 14q32.13.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1874-CAP | Anti-Human Serpina12 Pre-coated 96-well strip microplate | 1 |
| EK1874-ST | Human Serpina12 Standard | 2 vials, 10 ng/tube |
| EK1874-DA | Human Serpina12 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.025 | 0.160 | 0.263 | 0.491 | 0.852 | 1.290 | 1.878 | 2.305 |
Data Example Images
Click image to see more details
Human Serpin A12 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin A12, Dilution ratio of 1:10, concentration in serum and plasma is around 10 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Serpin A12 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 212 | 1109 | 5465 | 232 | 1235 | 6154 |
| Standard deviation | 11.24 | 68.76 | 245.93 | 10.90 | 79.04 | 332.32 |
| CV (%) | 5.3% | 6.2% | 4.5% | 4.7% | 6.4% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin A12 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 212 | 215 | 232 | 220 | 219 | 7.62 | 3.4% |
| Sample 2 | 1109 | 1215 | 1235 | 1149 | 1177 | 50.53 | 4.2% |
| Sample 3 | 5465 | 6356 | 5987 | 5976 | 5946 | 317.03 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For Serpina12 (Source: Uniprot.Org, NCBI)
Gene Name
Serpina12
Full Name
Serpin A12
Weight
47.634kDa
Superfamily
serpin family
Alternative Names
antitrypsin), member 12; member 12; OL-64; OL-64Visceral adipose-specific serpin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); Vaspin; Visceral adipose tissue-derived serine protease inhibitor Serpina12|4632419J12Rik|serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12|serpin A12|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12|vaspin|visceral adipose tissue-derived serine protease inhibitor|visceral adipose-specific SERPIN
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Serpina12, check out the Serpina12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Serpina12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Serpin A12 ELISA Kit PicoKine® (EK1874)
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No publications found for EK1874
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
9 Customer Q&As for Human Serpin A12 ELISA Kit PicoKine®
Question
Q: Can Serpin A12 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-12-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-12-03
Question
Q: which procedure should I follow in order to thaw whole blood sample for Serpin A12 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-10-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin A12 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-10-21
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-07-24
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-07-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-02-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-02-02
Question
Q: can I use citrate plasma as samples in Human Serpin A12 Picokine® ELISA Kit (Catalog # EK1874)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Serpin A12 causing disruption of its protein structure. Serpin A12 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Serpin A12 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-06-17
Question
Q: how do I analyze ELISA data? I measured Serpin A12 level in serum.
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-31
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
E. Moore
Verified customer
Asked: 2017-05-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-05-13
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Serpin A12 using the EK1874 Human Serpin A12 Picokine® ELISA Kit?
G. Li
Verified customer
Asked: 2017-05-01
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2017-05-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2014-07-03
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-07-03
