Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Overview
| Product Name |
Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit
See more Serpin C1/Antithrombin-III products |
| Catalog# |
FEK1393 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
The Quick version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Antithrombin Iii/SERPINC1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up. |
| Cite This Product |
Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1393)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
| Sensitivity |
<10pg/ml |
| Assay Range |
312pg/ml-20,000pg/ml |
| Immunogen/Standard |
NSO, H33-K464 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For SERPINC1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P01008 |
|---|
| NCBI Gene Id | 462 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | SERPINC1 |
|---|
| Protein Name | Antithrombin-III |
|---|
| Superfamily | serpin family |
|---|
| Alternative Names | Serpin C1/Antithrombin-III|Antithrombin-III; AT3antithrombin-III; ATIII; ATIIIantithrombin III; MGC22579; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serine-cysteine proteinase inhibitor clade C member 1; Serpin C1; serpin peptidase inhibitor, clade C (antithrombin), member 1 |
|---|
| Molecular Weight | 52602 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SERPINC1, check out the SERPINC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for SERPINC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the SERPINC1 infographic
Kit Components And Assay QC Data Details Of Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| FEK1393-CAP | 96-well plate precoated with anti-Human Serpin C1/Antithrombin-III antibody | 1 |
| FEK1393-ST | lyophilized recombinant Human Serpin C1/Antithrombin-III standard | 20ng/tube |
| FEK1393-DA | Biotinylated anti-Human Serpin C1/Antithrombin-III detection antibody (50x) | 130ul |
| - | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
| AR1106-1 | Sample diluent | 30ml |
| AR1106-2 | Antibody diluent | 12ml |
| - | Avidin-Biotin-Peroxidase Diluent | 12ml |
| - | Wash Buffer | powder pack for 1000ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| PLA-SEA | Plate Sealers | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of FEK1393 Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 25-30min
| Concentration(pg/ml) | 0 | 312 | 625 | 10,000000 | 10,00000 | 10,0000 | 10,000 | 20,000 |
| O.D. | 0.011 | 0.062 | 0.082 | 0.15 | 0.295 | 0.582 | 1.333 | 1.974 |
We measured random samples of Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 647 | 1973 | 8982 | 647 | 2050 | 9365 |
| Standard deviation | 28.46 | 153.89 | 377.24 | 34.29 | 174.25 | 552.53 |
| CV(%) | 4.4% | 7.8% | 4.2% | 5.3% | 8.5% | 5.9% |
We measured the performance consistency of Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 647 | 649 | 673 | 636 | 651 | 13.49 | 2% |
| Sample 2 | 1973 | 1798 | 1681 | 1827 | 1819 | 103.99 | 5.7% |
| Sample 3 | 8982 | 8247 | 8966 | 9377 | 8893 | 407.68 | 4.5% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Questions and answers from customers related to FEK1393 Human Serpin C1/Antithrombin-III PicoKine™ Quick ELISA Kit
1 Related Questions
Question
Just a question; for product FEK1393 in the Western Blot for antithrombin III; is that from cells or supernatant? If it is in the cell, which cells were used? Is there any blocking protein secretion used?
Verified Customer
Asked: 2016-09-19
Answer
The product FEK1393 have been tested successfully in cell lysates, which do not need to have protein secretion blocked. Additionally, there were enough non-secreted Antithrombin III still in the cytoplasm of the cells to give positive bands.
Boster Scientific Support
Answered: 2016-09-19
