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Product Info Summary
| SKU: | EK1221 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human soluble CD6 ELISA Kit PicoKine®
SKU/Catalog Number
EK1221
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human soluble CD6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human soluble CD6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1221)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H18-E398
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1221 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1221 is reactive to CD6 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1221 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD6
CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1221-CAP | Anti-Human CD6 Pre-coated 96-well strip microplate | 1 |
| EK1221-ST | Human CD6 Standard | 2 vials, 10 ng/tube |
| EK1221-DA | Human CD6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.002 | 0.056 | 0.108 | 0.219 | 0.416 | 0.728 | 1.202 | 1.845 |
Data Example Images
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Human soluble CD6 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human soluble CD6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 255 | 1450 | 4911 | 250 | 1511 | 5268 |
| Standard deviation | 14.79 | 72.5 | 378.14 | 16.5 | 75.55 | 447.78 |
| CV (%) | 5.8% | 5% | 7.7% | 6.6% | 5% | 8.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 255 | 257 | 218 | 221 | 237 | 18.29 | 7.7% |
| Sample 2 | 1450 | 1421 | 1416 | 1269 | 1389 | 70.48 | 5% |
| Sample 3 | 4911 | 4340 | 4503 | 4742 | 4624 | 218.85 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For CD6 (Source: Uniprot.Org, NCBI)
Gene Name
CD6
Full Name
T-cell differentiation antigen CD6
Weight
71.801kDa
Alternative Names
CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6 CD6 TP120 CD6 molecule T-cell differentiation antigen CD6|CD6 antigen|T12
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD6, check out the CD6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human soluble CD6 ELISA Kit PicoKine® (EK1221)
Hello CJ!
EK1221 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Multifaceted effects of soluble human CD6 in experimental cancer models
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human soluble CD6 ELISA Kit PicoKine®
Question
How are the Capture Antibody and Detection Antibody of EK1221 being purified?
Verified customer
Asked: 2019-10-28
Answer
Both the capture antibody and detection antibody of the Human Soluble CD6 ELISA Kit PicoKine® (EK1221) are Affinity purified.
Boster Scientific Support
Answered: 2019-10-28
Question
For all antibodies (include plate), what is the host and immunized animal for the capture and detection antibody of EK1221?
Verified customer
Asked: 2019-10-28
Answer
For the Human Soluble CD6 ELISA Kit PicoKine™ (EK1221), the host and immunized animal for the capture antibody is Goat. The host and immunized animal for the detection antibody is Goat.
Boster Scientific Support
Answered: 2019-10-29
Question
Does EK1221 contain preservatives (e.g. sodium azide, thimerosal), detergents (e.g. SDS, tritonX-100), alcohol (methanol, ethanol, propanol), other organic solvent (e.g. phenol, acetone etc.), acid (e.g. HCl, H2SO4 etc.), β-mercaptoethanol, Base (e.g. NaOH, KOH etc.) and Recombinant protein (If yes, please let us know the gene origin and expression system.)?
Verified customer
Asked: 2019-10-25
Answer
The Human Soluble CD6 ELISA Kit PicoKine™ (EK1221) contains a lyophilized recombinant Human CD6 standard Expression system for standard: NSO; Immunogen sequence: H18-E398
Boster Scientific Support
Answered: 2019-10-25
Question
Does EK1221 kit contain native human or monkey materials?
Verified customer
Asked: 2019-09-25
Answer
The Human soluble CD6 ELISA Kit PicoKine EK1221 kit contains no native human or monkey materials.
Boster Scientific Support
Answered: 2019-09-25
Question
Q: how do I analyze ELISA data? I have obtained CD6 level in cell lysates.
Verified Customer
Verified customer
Asked: 2017-02-09
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-02-09
Question
Q: if the enzyme conjugated CD6 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD6 antigen?
Verified Customer
Verified customer
Asked: 2016-05-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-05-12
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
G. Thomas
Verified customer
Asked: 2014-08-30
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-08-30
