Human soluble CD6 ELISA Kit PicoKine™

Human soluble CD6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1221
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human soluble CD6 ELISA Kit PicoKine™

See all CD6 products

SKU/Catalog Number

EK1221

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human soluble CD6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human soluble CD6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1221)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: H18-E398

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1221 is reactive to CD6 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1221 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1221-CAP Anti-Human CD6 Pre-coated 96-well strip microplate 1
EK1221-ST Human CD6 Standard 2 vials, 10 ng/tube
EK1221-DA Human CD6 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0020.1080.2190.4160.7281.2021.8452.546

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human soluble CD6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2551450491125015115268
Standard deviation14.7972.5378.1416.575.55447.78
CV (%)5.8%5%7.7%6.6%5%8.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD6 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 125525721822123718.297.7%
Sample 21450142114161269138970.485%
Sample 349114340450347424624218.854.7%
*number of samples for each test n=16.

Gene/Protein Information For CD6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P30203

Gene ID

923

Gene Name

CD6

Full Name

T-cell differentiation antigen CD6

Weight

71.801kDa

Alternative Names

CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6 CD6 TP120 CD6 molecule T-cell differentiation antigen CD6|CD6 antigen|T12

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD6, check out the CD6 Infographic

CD6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Human soluble CD6 ELISA Kit PicoKine™

Question

How are the Capture Antibody and Detection Antibody of EK1221 kit being purified?

Verified customer

Asked: 2019-10-28

Answer

Both the capture antibody and detection antibody of the Human soluble CD6 ELISA Kit PicoKine EK1221 are Affinity purified.

Boster Scientific Support

Answered: 2019-10-28

Question

For all antibodies (include plate), what is the host and immunized animal for the capture and detection antibody of EK1221?

Verified customer

Asked: 2019-10-28

Answer

For the Human Soluble CD6 ELISA Kit PicoKine™ (EK1221), the host and immunized animal for the capture antibody is Goat. The host and immunized animal for the detection antibody is Goat.

Boster Scientific Support

Answered: 2019-10-29

Question

Does EK1221 contain preservatives (e.g. sodium azide, thimerosal), detergents (e.g. SDS, tritonX-100), alcohol (methanol, ethanol, propanol), other organic solvent (e.g. phenol, acetone etc.), acid (e.g. HCl, H2SO4 etc.), β-mercaptoethanol, Base (e.g. NaOH, KOH etc.) and Recombinant protein (If yes, please let us know the gene origin and expression system.)?

Verified customer

Asked: 2019-10-25

Answer

The Human Soluble CD6 ELISA Kit PicoKine™ (EK1221) contains a lyophilized recombinant Human CD6 standard Expression system for standard: NSO; Immunogen sequence: H18-E398

Boster Scientific Support

Answered: 2019-10-25

Question

What is the recombinant protein and its expression system for EK1221 kit?

Verified customer

Asked: 2019-09-25

Answer

The Human soluble CD6 ELISA Kit PicoKine EK1221 contains a lyophilized recombinant Human CD6 standard. Expression system for standard: NSO; Immunogen sequence: H18-E398.

Boster Scientific Support

Answered: 2019-09-25

Question

Does EK1221 kit contain native human or monkey materials?

Verified customer

Asked: 2019-09-25

Answer

The Human soluble CD6 ELISA Kit PicoKine EK1221 kit contains no native human or monkey materials.

Boster Scientific Support

Answered: 2019-09-25

Question

Q: how do I analyze ELISA data? I have obtained CD6 level in cell lysates.

Verified Customer

Verified customer

Asked: 2017-02-09

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-02-09

Question

Q: if the enzyme conjugated CD6 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD6 antigen?

Verified Customer

Verified customer

Asked: 2016-05-12

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-05-12

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

G. Thomas

Verified customer

Asked: 2014-08-30

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2014-08-30

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