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Product Info Summary
| SKU: | EK0530 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. |
Product info
Product Name
Human sTNFsR II ELISA Kit PicoKine®
SKU/Catalog Number
EK0530
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human sTNFsR II ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF1B in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human sTNFsR II ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0530)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L23-D257
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<2 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
See datasheet of EK0530 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0530 is reactive to TNFRSF1B in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.
Application Guarantee
EK0530 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TNFRSF1B
Tumor necrosis factor receptor p75 (TNF-R p75, or TNF-R2) is a 75-kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II (TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0530-CAP | Anti-Human TNFRSF1B Pre-coated 96-well strip microplate | 1 |
| EK0530-ST | Human TNFRSF1B Standard | 2 vials, 10 ng/tube |
| EK0530-DA | Human TNFRSF1B Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.0 | 0.078 | 0.151 | 0.286 | 0.531 | 0.967 | 1.664 | 2.399 |
Data Example Images
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Human sTNFsR II PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TNFRSF1B, Dilution ratio of 1:20, concentration in serum and plasma is 802-3804 pg/ml..
Some articles we found to cite concentrations of TNFRSF1B in samples: 21403886 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human sTNFsR II ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 16 | 58 | 235 | 14 | 55 | 233 |
| Standard deviation | 1 | 2.43 | 15.04 | 0.89 | 3.19 | 15.37 |
| CV (%) | 5.8%% | 4.2% | 6.4% | 6.4% | 5.8% | 6.6% |
Specific Publications For Human sTNFsR II ELISA Kit PicoKine® (EK0530)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human sTNFsR II ELISA Kit PicoKine®
Question
Q: is it okay to use heparin plasma as samples in Human TNF RII Picokine® ELISA Kit (Catalog # EK0530)?
C. Brown
Verified customer
Asked: 2020-12-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TNF RII causing degradation of its protein structure. TNF RII may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TNF RII ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-12-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Kapoor
Verified customer
Asked: 2020-12-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-12-02
Question
Q: how can I thaw whole blood sample for TNF RII ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-08-25
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TNF RII for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-08-25
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-20
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2019-01-24
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TNF RII using the EK0530 Human TNF RII Picokine® ELISA Kit?
R. Moore
Verified customer
Asked: 2018-06-15
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-06-15
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-04-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-04-15
Question
Q: how to proceed with the analysis of ELISA data? I have performed TNF RII level in plasma.
Verified Customer
Verified customer
Asked: 2016-02-20
Answer
A: please read this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2016-02-20
