Human sTNFsR II ELISA Kit PicoKine™

Human sTNFsR II ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF1B in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 2pg/ml.

Product Info Summary

SKU: EK0530
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.

Product Name

Human sTNFsR II ELISA Kit PicoKine™

See all TNF RII/TNFRSF1B products

SKU/Catalog Number

EK0530

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human sTNFsR II ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF1B in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human sTNFsR II ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0530)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L23-D257

Sensitivity

<2 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0530 is reactive to TNFRSF1B in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.

Application Guarantee

EK0530 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0530-CAP Anti-Human TNFRSF1B Pre-coated 96-well strip microplate 1
EK0530-ST Human TNFRSF1B Standard 2 vials, 10 ng/tube
EK0530-DA Human TNFRSF1B Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.0370.0940.1450.2270.5320.8261.3102.021

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human sTNFsR II ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)16582351455233
Standard deviation12.4315.040.893.1915.37
CV (%)5.8%%4.2%6.4%6.4%5.8%6.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TNF RII/TNFRSF1B in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 116161716160.432.6%
Sample 258615856581.783%
Sample 323527422727525221.938.7%
*number of samples for each test n=16.

Gene/Protein Information For TNFRSF1B (Source: Uniprot.Org, NCBI)

Uniprot ID

P20333

Gene ID

7133

Gene Name

TNFRSF1B

Full Name

Tumor necrosis factor receptor superfamily member 1B

Weight

48.291kDa

Alternative Names

CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II TNFRSF1B CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR TNF receptor superfamily member 1B tumor necrosis factor receptor superfamily member 1B|TNF-R2|TNF-RII|p75 TNF receptor|p80 TNF-alpha receptor|soluble TNFR1B variant 1|tumor necrosis factor beta receptor|tumor necrosis factor binding protein 2|tumor necrosis factor receptor 2|tumor necrosis factor receptor type II

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF1B, check out the TNFRSF1B Infographic

TNFRSF1B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Human sTNFsR II ELISA Kit PicoKine™

Question

Q: is it okay to use heparin plasma as samples in Human TNF RII Picokine® ELISA Kit (Catalog # EK0530)?

C. Brown

Verified customer

Asked: 2020-12-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TNF RII causing degradation of its protein structure. TNF RII may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TNF RII ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-12-12

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

H. Kapoor

Verified customer

Asked: 2020-12-02

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-12-02

Question

Q: how can I thaw whole blood sample for TNF RII ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-08-25

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TNF RII for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-08-25

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-12-20

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-01-24

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-01-24

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TNF RII using the EK0530 Human TNF RII Picokine® ELISA Kit?

R. Moore

Verified customer

Asked: 2018-06-15

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-06-15

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-04-15

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-04-15

Question

Q: how to proceed with the analysis of ELISA data? I have performed TNF RII level in plasma.

Verified Customer

Verified customer

Asked: 2016-02-20

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-02-20

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