Human TIMP-2 ELISA Kit PicoKine™

Human TIMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. Cited in 8 publication(s).

Product Info Summary

SKU: EK0522
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Product Name

Human TIMP-2 ELISA Kit PicoKine™

See all TIMP-2 products

SKU/Catalog Number

EK0522

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TIMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TIMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0522)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: C27-P220

Sensitivity

<2 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0522 is reactive to TIMP2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EK0522 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0522-CAP Anti-Human TIMP2 Pre-coated 96-well strip microplate 1
EK0522-ST Human TIMP2 Standard 2 vials, 10 ng/tube
EK0522-DA Human TIMP2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0050.0510.1090.1980.4180.7871.2971.909

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TIMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2491786439923916544356
Standard deviation17.4394.65325.5216.73112.47361.54
CV (%)6.4%5.3%7.4%7%6.8%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TIMP-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 124925827529126816.116%
Sample 21786179817962014184895.655.1%
Sample 343995066450650164746297.196.2%
*number of samples for each test n=16.

Gene/Protein Information For TIMP2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P16035

Gene ID

7077

Gene Name

TIMP2

Full Name

Metalloproteinase inhibitor 2

Weight

24.399kDa

Superfamily

protease inhibitor I35 (TIMP) family

Alternative Names

CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2 TIMP2 CSC-21K, DDC8 TIMP metallopeptidase inhibitor 2 metalloproteinase inhibitor 2|testicular secretory protein Li 57|tissue inhibitor of metalloproteinases 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TIMP2, check out the TIMP2 Infographic

TIMP2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TIMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0522 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cosentino G,Di Stefano V,Presti RL,Montana M,Todisco M,Gastaldi M,Cortese A,Alfonsi E,Tassorelli C,Fierro B,Caimi G,Brighina F.Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy.Neurol Sci.2021 May 14.doi:10.1007/s10072-021-05314-y.Epub ahead of print.PMID:33988800.
Species: Human
EK0522 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Cavdar Z, Ozbal S, Celik A, Ergur Bu, Guneli E, Ural C, Camsari T, Guner Ga. Biotech Histochem. 2014 May;89(4):304-14. Doi: 10.3109/10520295.2013.847498. Epub 2013 Oct 28. The Effects Of Alpha-Lipoic Acid On Mmp-2 And Mmp-9 Activities In A Rat Ren...

Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon...

Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation.

Hopps E, Lo Presti R, Montana M, Noto D, Averna Mr, Caimi G. J Investig Med. 2013 Aug;61(6):978-83. Doi: 10.231/Jim.0B013E318294E9Da. Gelatinases And Their Tissue Inhibitors In A Group Of Subjects With Metabolic Syndrome.

Hopps E, Lo Presti R, Montana M, Canino B, Averna Mr, Caimi G. Mediators Inflamm. 2014;2014:510619. Doi: 10.1155/2014/510619. Epub 2014 Jul 10. Study Of The Correlations Among Some Parameters Of The Oxidative Status, Gelatinases, And Their Inhibit...

Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes.

Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.

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9 Customer Q&As for Human TIMP-2 ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for TIMP-2 ELISA kit? I used your TIMP-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TIMP-2 even though the O.D. values are not very high?

Z. Robinson

Verified customer

Asked: 2020-05-08

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TIMP-2 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of TIMP-2 that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2020-05-08

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

W. Hill

Verified customer

Asked: 2019-02-28

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-02-28

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TIMP-2 using the EK0522 Human TIMP-2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-11-10

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-11-10

Question

Q: how do I analyze ELISA data? I measured TIMP-2 level in serum.

D. Mehta

Verified customer

Asked: 2018-10-23

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-10-23

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-10-02

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-10-02

Question

Q: can I use citrate plasma as samples in Human TIMP-2 Picokine® ELISA Kit (Catalog # EK0522)?

R. Green

Verified customer

Asked: 2018-01-04

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TIMP-2 causing disruption of its protein structure. TIMP-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TIMP-2 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-01-04

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

J. Babu

Verified customer

Asked: 2018-01-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-01-03

Question

Q: how to thaw whole blood sample for TIMP-2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-11-26

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TIMP-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-11-26

Question

Q: if the enzyme conjugated TIMP-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TIMP-2 antigen?

A. Adams

Verified customer

Asked: 2016-07-04

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-07-04

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