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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.|
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Human TIMP-2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human TIMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TIMP-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0522)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: CHO; Immunogen sequence: C27-P220
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0522 is reactive to TIMP2 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
EK0522 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TIMP-2
TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
|EK0522-CAP||Anti-Human TIMP2 Pre-coated 96-well strip microplate||1|
|EK0522-ST||Human TIMP2 Standard||2 vials, 10 ng/tube|
|EK0522-DA||Human TIMP2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human TIMP-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TIMP-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TIMP-2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For TIMP2 (Source: Uniprot.Org, NCBI)
Metalloproteinase inhibitor 2
protease inhibitor I35 (TIMP) family
CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2 TIMP2 CSC-21K, DDC8 TIMP metallopeptidase inhibitor 2 metalloproteinase inhibitor 2|testicular secretory protein Li 57|tissue inhibitor of metalloproteinases 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TIMP2, check out the TIMP2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TIMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0522 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gelatinases and their tissue inhibitors in a group of subjects with obstructive sleep apnea syndrome
Behaviour of the plasma concentration of gelatinases and their tissue inhibitors in subjects with venous leg ulcers
Gelatinases and Their Tissue Inhibitors in a Group of Subjects With Metabolic Syndrome
MMP-2, MMP-9, TIMP-1, and TIMP-2 in the Peripheral Blood of Patients with Differentiated Thyroid Carcinoma
Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy
Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13
Cosentino G,Di Stefano V,Presti RL,Montana M,Todisco M,Gastaldi M,Cortese A,Alfonsi E,Tassorelli C,Fierro B,Caimi G,Brighina F.Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy.Neurol Sci.2021 May 14.doi:10.1007/s10072-021-05314-y.Epub ahead of print.PMID:33988800.
EK0522 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Cavdar Z, Ozbal S, Celik A, Ergur Bu, Guneli E, Ural C, Camsari T, Guner Ga. Biotech Histochem. 2014 May;89(4):304-14. Doi: 10.3109/10520295.2013.847498. Epub 2013 Oct 28. The Effects Of Alpha-Lipoic Acid On Mmp-2 And Mmp-9 Activities In A Rat Ren...
Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon...
Yu X, Zhu J, Mi M, Chen W, Pan Q, Wei M. Med Oncol. 2012 Mar;29(1):349-57. Doi: 10.1007/S12032-010-9770-2. Epub 2010 Dec 4. Anti-Angiogenic Genistein Inhibits Vegf-Induced Endothelial Cell Activation By Decreasing Ptk Activity And Mapk Activation.
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