Human TNFRSF21/DR6 ELISA Kit PicoKine®

DR6/TNFRSF21 ELISA kit for Human

Human TNFRSF21/DR6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF21 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK2004
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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Product Name

Human TNFRSF21/DR6 ELISA Kit PicoKine®

View all DR6/TNFRSF21 ELISA kits

SKU/Catalog Number

EK2004

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TNFRSF21/DR6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF21 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TNFRSF21/DR6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2004)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q42-H349

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2004 is reactive to TNFRSF21 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2004 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of DR6/TNFRSF21

Death receptor 6 (DR6), also known as tumor necrosis factor receptor superfamily member 21 (TNFRSF21), is a cell surface receptor of the tumor necrosis factor receptor superfamily which activates the JNK and NF-κB pathways. This gene is mapped to 6p12.3. This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1), and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation.

Kit Components

Catalog Number Description Quantity
EK2004-CAP Anti-Human TNFRSF21 Pre-coated 96-well strip microplate 1
EK2004-ST Human TNFRSF21 Standard 2 vials, 10 ng/tube
EK2004-DA Human TNFRSF21 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0330.0810.1300.2200.4080.7931.3922.219

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect DR6/TNFRSF21, Dilution ratio of 1:1, concentration in serum is around 30pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TNFRSF21/DR6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)219437826103431
Standard deviation0.865.8317.011.226.5923.27
CV (%)4.1%6.2%4.5%4.7%6.4%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of DR6/TNFRSF21 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 121222523221.476.6%
Sample 2949110799976.056.2%
Sample 337840241538839514.003.5%
*number of samples for each test n=16.

Gene/Protein Information For TNFRSF21 (Source: Uniprot.Org, NCBI)

Uniprot ID

O75509

Gene ID

27242

Gene Name

TNFRSF21

Full Name

Tumor necrosis factor receptor superfamily member 21

Weight

71.845kDa

Alternative Names

BM-018; CD358 antigen; CD358; Death receptor 6; DR6; DR6TNFR-related death receptor 6; MGC31965; TNFRSF21; tumor necrosis factor receptor superfamily member 21; tumor necrosis factor receptor superfamily, member 21 TNFRSF21 BM-018, CD358, DR6 TNF receptor superfamily member 21 tumor necrosis factor receptor superfamily member 21|TNFR-related death receptor 6|death receptor 6

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF21, check out the TNFRSF21 Infographic

TNFRSF21 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human TNFRSF21/DR6 ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Lopez

Verified customer

Asked: 2019-03-27

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-03-27

Question

Q: if the enzyme conjugated DR6 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DR6 antigen?

Y. Edwards

Verified customer

Asked: 2018-11-28

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-11-28

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

A. Harris

Verified customer

Asked: 2018-08-13

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-08-13

Question

Q: What is the optimal O.D. value for DR6 ELISA kit? I performed your DR6 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain DR6 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-07-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this DR6 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of DR6 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-07-27

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2016-12-05

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-12-05

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-01-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-01-25

Question

Q: is it okay to use EDTA plasma as samples in Human DR6 Picokine® ELISA Kit (Catalog # EK2004)?

Verified Customer

Verified customer

Asked: 2015-09-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of DR6 causing disruption of its protein structure. DR6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the DR6 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2015-09-12

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