Product Info Summary
SKU: | EK2004 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human TNFRSF21/DR6 ELISA Kit PicoKine®
View all DR6/TNFRSF21 ELISA kits
SKU/Catalog Number
EK2004
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFRSF21/DR6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF21 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFRSF21/DR6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2004)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q42-H349
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2004 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2004 is reactive to TNFRSF21 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2004 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DR6/TNFRSF21
Death receptor 6 (DR6), also known as tumor necrosis factor receptor superfamily member 21 (TNFRSF21), is a cell surface receptor of the tumor necrosis factor receptor superfamily which activates the JNK and NF-κB pathways. This gene is mapped to 6p12.3. This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1), and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2004-CAP | Anti-Human TNFRSF21 Pre-coated 96-well strip microplate | 1 |
EK2004-ST | Human TNFRSF21 Standard | 2 vials, 10 ng/tube |
EK2004-DA | Human TNFRSF21 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.033 | 0.081 | 0.130 | 0.220 | 0.408 | 0.793 | 1.392 | 2.219 |
Data Example Images
Click image to see more details
Human TNFRSF21/DR6 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect DR6/TNFRSF21, Dilution ratio of 1:1, concentration in serum and plasma is around 40 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFRSF21/DR6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 21 | 94 | 378 | 26 | 103 | 431 |
Standard deviation | 0.86 | 5.83 | 17.01 | 1.22 | 6.59 | 23.27 |
CV (%) | 4.1% | 6.2% | 4.5% | 4.7% | 6.4% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DR6/TNFRSF21 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 21 | 22 | 25 | 23 | 22 | 1.47 | 6.6% |
Sample 2 | 94 | 91 | 107 | 99 | 97 | 6.05 | 6.2% |
Sample 3 | 378 | 402 | 415 | 388 | 395 | 14.00 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF21 (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF21
Full Name
Tumor necrosis factor receptor superfamily member 21
Weight
71.845kDa
Alternative Names
BM-018; CD358 antigen; CD358; Death receptor 6; DR6; DR6TNFR-related death receptor 6; MGC31965; TNFRSF21; tumor necrosis factor receptor superfamily member 21; tumor necrosis factor receptor superfamily, member 21 TNFRSF21 BM-018, CD358, DR6 TNF receptor superfamily member 21 tumor necrosis factor receptor superfamily member 21|TNFR-related death receptor 6|death receptor 6
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF21, check out the TNFRSF21 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TNFRSF21/DR6 ELISA Kit PicoKine® (EK2004)
Hello CJ!
No publications found for EK2004
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human TNFRSF21/DR6 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Human TNFRSF21/DR6 ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human TNFRSF21/DR6 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Lopez
Verified customer
Asked: 2019-03-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-03-27
Question
Q: if the enzyme conjugated DR6 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DR6 antigen?
Y. Edwards
Verified customer
Asked: 2018-11-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-28
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
A. Harris
Verified customer
Asked: 2018-08-13
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-08-13
Question
Q: What is the optimal O.D. value for DR6 ELISA kit? I performed your DR6 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain DR6 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-07-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this DR6 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of DR6 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-07-27
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-12-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-12-05
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-01-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-01-25
Question
Q: is it okay to use EDTA plasma as samples in Human DR6 Picokine® ELISA Kit (Catalog # EK2004)?
Verified Customer
Verified customer
Asked: 2015-09-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of DR6 causing disruption of its protein structure. DR6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the DR6 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-09-12