Human TNFRSF7/CD27 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF7/CD27. 96wells/kit, with removable strips.
Human TNFRSF7/CD27 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates and serum.|
|Sample Volume:||100μl per well|
|Product Name||Human TNFRSF7/CD27 ELISA Kit PicoKine™
See all CD27 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Tnfrsf7/CD27 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Tnfrsf7/CD27 tissue specificity: Found in most T-lymphocytes.|
|Cite This Product||Human TNFRSF7/CD27 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0698)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates and serum.|
|Immunogen||Expression system for standard: NSO; Immunogen sequence: T21-I192|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||CD27 antigen|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Human TNFRSF7/CD27 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0698-CAP||96-well plate precoated with anti-Human CD27 antibody||1|
|EK0698-ST||lyophilized recombinant Human CD27 standard||10ng/tube|
|EK0698-DA||biotinylated anti-Human CD27 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0698 Human TNFRSF7/CD27 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human TNFRSF7/CD27 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human TNFRSF7/CD27 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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