Human TRAIL / CD253 ELISA Kit PicoKine™

Human TRAIL / CD253 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml. Cited in 4 publication(s).

Product Info Summary

SKU: EK0532
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.

Product Name

Human TRAIL / CD253 ELISA Kit PicoKine™

See all TRAIL/TNFSF10 products

SKU/Catalog Number

EK0532

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TRAIL / CD253 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TRAIL / CD253 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0532)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T95-G281

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0532 is reactive to TNFSF10 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EK0532 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0532-CAP Anti-Human TNFSF10 Pre-coated 96-well strip microplate 1
EK0532-ST Human TNFSF10 Standard 2 vials, 10 ng/tube
EK0532-DA Human TNFSF10 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0260.0780.1110.1950.3770.6591.1661.912

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TRAIL / CD253 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3818441634175377
Standard deviation2.669.5618.722.759.6218.85
CV (%)7%5.2%4.5%8.1%5.5%5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TRAIL/TNFSF10 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 138353542372.877.7%
Sample 21841871851891861.921%
Sample 341646939839041830.777.3%
*number of samples for each test n=16.

Gene/Protein Information For TNFSF10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50591

Gene ID

8743

Gene Name

TNFSF10

Full Name

Tumor necrosis factor ligand superfamily member 10

Weight

32.509kDa

Superfamily

tumor necrosis factor family

Alternative Names

Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10 TNFSF10 APO2L, Apo-2L, CD253, TL2, TNLG6A, TRAIL TNF superfamily member 10 tumor necrosis factor ligand superfamily member 10|Apo-2 ligand|TNF-related apoptosis inducing ligand TRAIL|chemokine tumor necrosis factor ligand superfamily member 10|tumor necrosis factor (ligand) family, member 10|tumor necrosis factor (ligand) superfamily, member 10|tumor necrosis factor apoptosis-inducing ligand splice variant delta|tumor necrosis factor ligand 6A|tumor necrosis factor superfamily member 10

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFSF10, check out the TNFSF10 Infographic

TNFSF10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFSF10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0532 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Engineering bacterial outer membrane vesicles as transdermal nanoplatforms for photo-TRAIL–programmed therapy against melanoma
Species: Human

A novel anti-GD2/4-1BB chimeric antigen receptor triggers neuroblastoma cell killing

Sun R, Zhang Y, Lv Q, Liu B, Jin M, Zhang W, He Q, Deng M, Liu X, Li G, Li Y, Zhou G, Xie P, Xie X, Hu J, Duan Z. J Biol Chem. 2011 May 6;286(18):15918-28. Doi: 10.1074/Jbc.M110.178798. Epub 2011 Mar 2. Toll-Like Receptor 3 (Tlr3) Induces Apoptosi...

Duan Y, Gu X, Zhu D, Sun W, Chen J, Feng J, Song K, Xu F, He X, He X. Int J Parasitol. 2014 Mar;44(3-4):217-24. Doi: 10.1016/J.Ijpara.2013.11.003. Epub 2014 Jan 31. Schistosoma Japonicum Soluble Egg Antigens Induce Apoptosis And Inhibit Activation...

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Have a question?

Find answers in Q&As, reviews.

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9 Customer Q&As for Human TRAIL / CD253 ELISA Kit PicoKine™

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TRAIL in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

E. Martin

Verified customer

Asked: 2020-10-22

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TRAIL ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-10-22

Question

Q: which procedure should I follow in order to thaw whole blood sample for TRAIL ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-09-23

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TRAIL for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-09-23

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-07-06

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-07-06

Question

Q: if the enzyme conjugated TRAIL antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TRAIL antigen?

P. Nelson

Verified customer

Asked: 2020-03-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-03-09

Question

TRAIL has two isoforms, and Isoform 2 differs from Isoform 1 because it is missing the first 180 amino acids of the ECM domain. Do you know if this ELISA will detect both Isoform 1 and isoform 2? Keyword: detection

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

The kit can detect both isoform 1 and isoform 2.

Boster Scientific Support

Answered: 2020-02-28

Question

In the manual, in the section assay procedure, step 2 it is written: "Seal the plate with the cover and incubate at 37°C for 90 min." In the kit components an adhesive seal wasn't included. What is your recommendation on how or with what I should seal the plate? Keyword: contamination, lid

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

The seal is mainly to prevent contamination. It is not a crucial step/component to the experiment. If the customer does not have any cover or seal, it is OK to place a piece of paper over it--as long as the cover does not contain the detection target to influence the measurement. It is OK to not cover it too, especially when the development only takes less than 30 minutes--some ELISA kits develop faster than others.

Boster Scientific Support

Answered: 2019-06-26

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-11-27

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-11-27

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-01-30

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-01-30

Question

Q: Can TRAIL ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-01-09

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