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Human TRAIL / CD253 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.. Sensitivity: 1pg/ml. Cited in 3 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.|
|Sample Volume:||100μl per well|
|Assay Range:||15.6 pg/ml - 1,000 pg/ml|
|Product Name||Human TRAIL / CD253 ELISA Kit PicoKine™
See more TRAIL/TNFSF10 products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Human TRAIL / CD253 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.. Sensitivity: 1pg/ml.|
|Cite This Product||Human TRAIL / CD253 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0532)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.|
|Assay Range||15.6 pg/ml - 1,000 pg/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: T95-G281|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||8743|
|Species Of This Entry||Human|
|Protein Name||Tumor necrosis factor ligand superfamily member 10|
|Superfamily||tumor necrosis factor family|
|Alternative Names||TRAIL/TNFSF10|Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for TNFSF10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0532-CAP||Anti-Human TNFSF10 Pre-coated 96-well strip microplate||1|
|EK0532-ST||Human TNFSF10 Standard||2 vials, 10 ng/tube|
|EK0532-DA||Human TNFSF10 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0532 Human TRAIL / CD253 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human TRAIL / CD253 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TRAIL/TNFSF10 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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|A novel anti-GD2/4-1BB chimeric antigen receptor triggers neuroblastoma cell killing||PubMed ID 26298772|
|Sun R, Zhang Y, Lv Q, Liu B, Jin M, Zhang W, He Q, Deng M, Liu X, Li G, Li Y, Zhou G, Xie P, Xie X, Hu J, Duan Z. J Biol Chem. 2011 May 6;286(18):15918-28. Doi: 10.1074/Jbc.M110.178798. Epub 2011 Mar 2. Toll-Like Receptor 3 (Tlr3) Induces Apoptosi...||PubMed ID 21367858|
|Duan Y, Gu X, Zhu D, Sun W, Chen J, Feng J, Song K, Xu F, He X, He X. Int J Parasitol. 2014 Mar;44(3-4):217-24. Doi: 10.1016/J.Ijpara.2013.11.003. Epub 2014 Jan 31. Schistosoma Japonicum Soluble Egg Antigens Induce Apoptosis And Inhibit Activation...||PubMed ID 24487000|
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Comprehensive info on TNFSF10, beautifully presented.Open Infographic
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