Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine™

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KDR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml. Cited in 4 publication(s).

Product Info Summary

SKU: EK0544
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine™

See all VEGFR2/KDR/Flk-1 products

SKU/Catalog Number

EK0544

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KDR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0544)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-E764

Sensitivity

<4 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0544 is reactive to KDR in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0544 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0544-CAP Anti-Human KDR Pre-coated 96-well strip microplate 1
EK0544-ST Human KDR Standard 2 vials, 10 ng/tube
EK0544-DA Human KDR Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0650.1150.1650.2650.4460.8141.5172.462

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)17740017621674101810
Standard deviation10.7926137.4311.5229.52161.09
CV (%)6.1%6.5%7.8%6.9%7.2%8.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of VEGFR2/KDR/Flk-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11771801691661735.73.2%
Sample 2400400444444422225.2%
Sample 31762165218181564169998.185.7%
*number of samples for each test n=16.

Gene/Protein Information For KDR (Source: Uniprot.Org, NCBI)

Uniprot ID

P35968

Gene ID

3791

Gene Name

KDR

Full Name

Vascular endothelial growth factor receptor 2

Weight

151.527kDa

Superfamily

protein kinase superfamily

Alternative Names

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KDR, check out the KDR Infographic

KDR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KDR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0544 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Role of the HIF%u20111 signaling pathway in chronic obstructive pulmonary disease

eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation

Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene

Wang L, Liu X, Wang H, Wang S. Oncol Lett. 2013 Jul;6(1):175-180. Epub 2013 May 15. Correlation Of The Expression Of Vascular Endothelial Growth Factor And Its Receptors With Microvessel Density In Ovarian Cancer.

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6 Customer Q&As for Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine™

Question

Q: is it okay to use citrate plasma as samples in Human VEGFR2 Picokine® ELISA Kit (Catalog # EK0544)?

C. Yang

Verified customer

Asked: 2019-11-03

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of VEGFR2 causing disruption of its protein structure. VEGFR2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the VEGFR2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-11-03

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

P. Jackson

Verified customer

Asked: 2019-10-03

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-10-03

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

W. Bhasin

Verified customer

Asked: 2019-03-04

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-03-04

Question

Q: how to proceed with the analysis of ELISA data? I have obtained VEGFR2 level in plasma.

Verified Customer

Verified customer

Asked: 2018-06-22

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-06-22

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGFR2 using the EK0544 Human VEGFR2 Picokine® ELISA Kit?

M. Williams

Verified customer

Asked: 2018-03-04

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2018-03-04

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-05-12

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-05-12

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