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Product Info Summary
| SKU: | PROTP56704 |
|---|---|
| Size: | Starting from 20ug |
| Source: | E. coli |
| Application: | SDS-PAGE, Denatured |
Product info
Product Name
Human WNT3a Recombinant Protein
View all WNT3A recombinant proteins
SKU/Catalog Number
PROTP56704
Size
Starting from 20ug
Tag
Non-Tagged
Description
Human WNT3a Recombinant Protein expressed in E. coli (non-tagged). Sequence domain: 19-352aa. Application(s): SDS-PAGE, Denatured. Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human WNT3a Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP56704)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Concentration
0.5mg/ml (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Predicted MW
39.4 kDa
Amino Acid Sequence
Human, P56704, 19-352aa; MSYPIWWSLA VGPQYSSLGS QPILCASIPG LVPKQLRFCR NYVEIMPSVA EGIKIGIQEC QHQFRGRRWN CTTVHDSLAI FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTAAICG CSSRHQGSPG KGWKWGGCSE DIEFGGMVSR EFADARENRP DARSAMNRHN NEAGRQAIAS HMHLKCKCHG LSGSCEVKTC WWSQPDFRAI GDFLKDKYDS ASEMVVEKHR ESRGWVETLR PRYTYFKVPT ERDLVYYEAS PNFCEPNPET GSFGTRDRTC NVSSHGIDGC DLLCCGRGHN ARAERRREKC RCVFHWCCYV SCQECTRVYD VHTCKAssay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For Human WNT3a Recombinant Protein (PROTP56704)
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1 Customer Q&As for Human WNT3a Recombinant Protein
Question
What is the difference between your PROTP56704 protein and Time Bioscience rhW3aH protein?
Verified customer
Asked: 2019-11-06
Answer
The following are the differences between Boster's and TimeBioscience's recombinant proteins. Boster. Purity: Greater than 85.0% as determined by SDS-PAGE. Sequence: MSYPIWWSLA VGPQYSSLGS QPILCASIPG LVPKQLRFCR NYVEIMPSVA EGIKIGIQEC QHQFRGRRWN CTTVHDSLAI FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTAAICG CSSRHQGSPG KGWKWGGCSE DIEFGGMVSR EFADARENRP DARSAMNRHN NEAGRQAIAS HMHLKCKCHG LSGSCEVKTC WWSQPDFRAI GDFLKDKYDS ASEMVVEKHR ESRGWVETLR PRYTYFKVPT ERDLVYYEAS PNFCEPNPET GSFGTRDRTC NVSSHGIDGC DLLCCGRGHN ARAERRREKC RCVFHWCCYV SCQECTRVYD VHTCK. Activity: We have not assessed the bio-functionality of the protein to date. So, we cannot guarantee that the protein is biologically active. Formulation: The WNT3A Human protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. Soruce: Escherichia Coli. TimeBioscience. Purity: ~ 75 % evaluated by SDS-PAGE under reducing conditions Sequences: Ser19-Lys352 Activity: Wnt3a activity has been measured using TCFbased Wnt reporter stable cell line (Catalog WRHEK293A-HWR).10 ng/mL of Wnt3a (Lot: 22JAN2016) generate 50-fold increase of luciferase activity compared to control (buffer without Wnt3a). Formulation: Phosphate buffer, pH 7.4-7.6, CHAPS, 0.1% BSA Source: Human cell-derived
Boster Scientific Support
Answered: 2019-11-06
