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Product Info Summary
| SKU: | LS055764 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2 |
Product info
Product Name
IFT122 (NM_052989) Human Over-expression Lysate
SKU/Catalog Number
LS055764
Size
100 µg
Description
Transient overexpression lysate of intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2
Storage & Handling
Cite This Product
IFT122 (NM_052989) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS055764)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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IFT122 Human Over-expression Lysates NM_052989.
Western blot validation of overexpression lysate (LS055764) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IFT122 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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IFT122 Human Over-expression Lysates NM_052989.
Western blot validation of overexpression lysate (LS055764) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IFT122 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
IFT122 Human Over-expression Lysates NM_052990.
Western blot validation of overexpression lysate (LS055764) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IFT122 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IFT122 (Source: Uniprot.org, NCBI)
Gene Name
IFT122
Full Name
Intraflagellar transport protein 122 homolog
Weight
141.825kDa
Alternative Names
intraflagellar transport 122 homolog (Chlamydomonas); intraflagellar transport protein 122 homolog; SPGWD repeat-containing protein 140; WD repeat domain 10; WD repeat-containing protein 10; WDR10WDR10p; WDR140CED IFT122 CED, CED1, FAP80, SPG, WDR10, WDR10p, WDR140 intraflagellar transport 122 intraflagellar transport protein 122 homolog|WD repeat domain 10|WD repeat-containing protein 10|WD repeat-containing protein 140|intraflagellar transport 122 homolog
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IFT122, check out the IFT122 Infographic
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In this infographic, you will see the following information for IFT122: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IFT122 (NM_052989) Human Over-expression Lysate (LS055764)
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