IGFBP3 (NM_001013398) Human Over-expression Lysate

Lysate of IGFBP-3 over-expression cell lines, WB control

Transient overexpression lysate of insulin-like growth factor binding protein 3 (IGFBP3), transcript variant 1

Product Info Summary

SKU: LS003486
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of insulin-like growth factor binding protein 3 (IGFBP3), transcript variant 1

Product Name

IGFBP3 (NM_001013398) Human Over-expression Lysate

See all IGFBP-3 products

SKU/Catalog Number

LS003486

Size

100 µg

Description

Transient overexpression lysate of insulin-like growth factor binding protein 3 (IGFBP3), transcript variant 1

Storage & Handling

Cite This Product

IGFBP3 (NM_001013398) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003486)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for IGFBP3 (Source: Uniprot.org, NCBI)

Gene Name

IGFBP3

Full Name

Insulin-like growth factor-binding protein 3

Weight

31.674kDa

Alternative Names

acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3 IGFBP3 BP-53, IBP3 insulin like growth factor binding protein 3 insulin-like growth factor-binding protein 3|IBP-3|IGF-binding protein 3|IGFBP-3|acid stable subunit of the 140 K IGF complex|binding protein 29|binding protein 53|growth hormone-dependent binding protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on IGFBP3, check out the IGFBP3 Infographic

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