IL1F10 (NM_032556) Human Over-expression Lysate

Lysate of IL-38/IL-1F10 over-expression cell lines, WB control

Transient overexpression lysate of interleukin 1 family, member 10 (theta) (IL1F10), transcript variant 1

Product Info Summary

SKU: LS084639
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of interleukin 1 family, member 10 (theta) (IL1F10), transcript variant 1

Product Name

IL1F10 (NM_032556) Human Over-expression Lysate

See all IL-38/IL-1F10 products

SKU/Catalog Number

LS084639

Size

100 µg

Description

Transient overexpression lysate of interleukin 1 family, member 10 (theta) (IL1F10), transcript variant 1

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

IL1F10 (NM_032556) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084639)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for IL1F10 (Source: Uniprot.org, NCBI)

Gene Name

IL1F10

Full Name

Interleukin-1 family member 10

Weight

16.943kDa

Superfamily

IL-1 family

Alternative Names

FIL1- theta; FKSG75; IL1F10; IL-1HY2; IL38; IL-38; interleukin 1 family, member 10 (theta); UNQ6119/PRO20041 IL1F10 FIL1-theta, FKSG75, IL-1HY2, IL-38, IL1-theta, IL1HY2 interleukin 1 family member 10 interleukin-1 family member 10|FIL1 theta|IL-1 theta|IL-1F10 (canonical form IL-1F10a)|family of interleukin 1-theta|interleukin 1 family member 10 (theta)|interleukin-1 HY2|interleukin-1 receptor antagonist FKSG75|interleukin-1 receptor antagonist-like FIL1 theta|interleukin-38

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on IL1F10, check out the IL1F10 Infographic

IL1F10 infographic

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In this infographic, you will see the following information for IL1F10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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