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Product Info Summary
| SKU: | LS051561 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of interleukin 23, alpha subunit p19 (IL23A) |
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Product info
Product Name
IL23 (IL23A) (NM_016584) Human Over-expression Lysate
See all IL-23A/IL-23 P19 products
SKU/Catalog Number
LS051561
Size
100 µg
Description
Transient overexpression lysate of interleukin 23, alpha subunit p19 (IL23A)
Storage & Handling
Cite This Product
IL23 (IL23A) (NM_016584) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS051561)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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IL23 (IL23A) Human Over-expression Lysates NM_016584.
Western blot validation of overexpression lysate (LS051561) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IL23A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IL23A (Source: Uniprot.org, NCBI)
Gene Name
IL23A
Full Name
Interleukin-23 subunit alpha
Weight
20.73kDa
Superfamily
IL-6 superfamily
Alternative Names
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha IL23A IL-23, IL-23A, IL23P19, P19, SGRF interleukin 23 subunit alpha interleukin-23 subunit alpha|IL-23 subunit alpha|IL-23-A|IL-23p19|JKA3 induced upon T-cell activation|interleukin 23 p19 subunit|interleukin 23, alpha subunit p19|interleukin-23 subunit p19|interleukin-six, G-CSF related factor
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL23A, check out the IL23A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL23A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IL23 (IL23A) (NM_016584) Human Over-expression Lysate (LS051561)
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No publications found for LS051561
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