IMMP2L (NM_032549) Human Over-expression Lysate

Lysate of IMMP2L over-expression cell lines, WB control

Transient overexpression lysate of IMP2 inner mitochondrial membrane peptidase-like (S. cerevisiae) (IMMP2L), nuclear gene encoding mitochondrial protein

Product Info Summary

SKU: LS083943
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of IMP2 inner mitochondrial membrane peptidase-like (S. cerevisiae) (IMMP2L), nuclear gene encoding mitochondrial protein

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Product Name

IMMP2L (NM_032549) Human Over-expression Lysate

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SKU/Catalog Number

LS083943

Size

100 µg

Description

Transient overexpression lysate of IMP2 inner mitochondrial membrane peptidase-like (S. cerevisiae) (IMMP2L), nuclear gene encoding mitochondrial protein

Storage & Handling

Cite This Product

IMMP2L (NM_032549) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083943)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for IMMP2L (Source: Uniprot.org, NCBI)

Gene Name

IMMP2L

Full Name

Mitochondrial inner membrane protease subunit 2

Weight

19.718kDa

Superfamily

peptidase S26 family

Alternative Names

Mitochondrial inner membrane protease subunit 2 IMMP2L IMMP2L-IT1, IMP2, IMP2-LIKE inner mitochondrial membrane peptidase subunit 2 mitochondrial inner membrane protease subunit 2|IMP2 inner mitochondrial membrane peptidase-like|IMP2 inner mitochondrial membrane protease-like|inner mitochondrial membrane peptidase 2 like

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on IMMP2L, check out the IMMP2L Infographic

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