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Product Info Summary
|Description:||Transient overexpression lysate of IMP2 inner mitochondrial membrane peptidase-like (S. cerevisiae) (IMMP2L), nuclear gene encoding mitochondrial protein|
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IMMP2L (NM_032549) Human Over-expression Lysate
Transient overexpression lysate of IMP2 inner mitochondrial membrane peptidase-like (S. cerevisiae) (IMMP2L), nuclear gene encoding mitochondrial protein
Storage & Handling
Cite This Product
IMMP2L (NM_032549) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083943)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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IMMP2L Human Over-expression Lysates NM_032549.
Western blot validation of overexpression lysate (LS083943) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IMMP2L (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for IMMP2L (Source: Uniprot.org, NCBI)
Mitochondrial inner membrane protease subunit 2
peptidase S26 family
Mitochondrial inner membrane protease subunit 2 IMMP2L IMMP2L-IT1, IMP2, IMP2-LIKE inner mitochondrial membrane peptidase subunit 2 mitochondrial inner membrane protease subunit 2|IMP2 inner mitochondrial membrane peptidase-like|IMP2 inner mitochondrial membrane protease-like|inner mitochondrial membrane peptidase 2 like*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on IMMP2L, check out the IMMP2L Infographic
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