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Product Info Summary
|Description:||Transient overexpression lysate of INO80 complex subunit B (INO80B)|
INO80B (NM_031288) Human Over-expression Lysate
Transient overexpression lysate of INO80 complex subunit B (INO80B)
Storage & Handling
Cite This Product
INO80B (NM_031288) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083444)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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INO80B Human Over-expression Lysates NM_031288.
Western blot validation of overexpression lysate (LS083444) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with INO80B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for INO80B (Source: Uniprot.org, NCBI)
INO80 complex subunit B
HMGIYL4; IES2 homolog; IES2; INO80 complex subunit B; PAP-1 binding protein; PAP-1BP; PAPA1; zinc finger, HIT type 4 INO80B HMGA1L4, HMGIYL4, IES2, PAP-1BP, PAPA-1, PAPA1, ZNHIT4, hIes2 INO80 complex subunit B INO80 complex subunit B|IES2 homolog|PAP (Pim-1 associated protein) associated protein 1|PAP-1 binding protein|PAP-1-associated protein 1|high mobility group AT-hook 1-like 4|zinc finger HIT domain-containing protein 4|zinc finger, HIT type 4*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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