INPP5A (NM_005539) Human Over-expression Lysate

Lysate of INPP5A over-expression cell lines, WB control

Transient overexpression lysate of inositol polyphosphate-5-phosphatase, 40kDa (INPP5A)

Product Info Summary

SKU: LS003632
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of inositol polyphosphate-5-phosphatase, 40kDa (INPP5A)

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Product Name

INPP5A (NM_005539) Human Over-expression Lysate

See all INPP5A products

SKU/Catalog Number

LS003632

Size

100 µg

Description

Transient overexpression lysate of inositol polyphosphate-5-phosphatase, 40kDa (INPP5A)

Storage & Handling

Cite This Product

INPP5A (NM_005539) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003632)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for INPP5A (Source: Uniprot.org, NCBI)

Gene Name

INPP5A

Full Name

Inositol polyphosphate-5-phosphatase A

Weight

47.82kDa

Superfamily

inositol 1,4,5-trisphosphate 5-phosphatase type I family

Alternative Names

43 kDa inositol polyphosphate 5-phophatase; CTCL tumor antigen HD-CL-02,5PTASE; DKFZp434A1721; EC 3.1.3.56; inositol polyphosphate-5-phosphatase, 40kD; inositol polyphosphate-5-phosphatase, 40kDa; inositol trisphosphate-5-phosphatase, 40kD; InsP3 5-phosphatase; MGC116947,5PTase; MGC116949; type I inositol-14,5-trisphosphate 5-phophatase; type I inositol-14,5-trisphosphate 5-phosphatase INPP5A 5PTASE inositol polyphosphate-5-phosphatase A inositol polyphosphate-5-phosphatase A|43 kDa inositol polyphosphate 5-phophatase|43 kDa inositol polyphosphate 5-phosphatase|CTCL tumor antigen HD-CL-02|InsP3 5-phosphatase|inositol polyphosphate-5-phosphatase, 40kD|inositol polyphosphate-5-phosphatase, 40kDa|inositol trisphosphate-5-phosphatase, 40kD|type I inositol 1,4,5-trisphosphate 5-phosphatase|type I inositol-1,4,5-trisphosphate 5-phophatase

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on INPP5A, check out the INPP5A Infographic

INPP5A infographic

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In this infographic, you will see the following information for INPP5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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