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Product Info Summary
| SKU: | LS002648 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of K(lysine) acetyltransferase 2A (KAT2A) |
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Product info
Product Name
KAT2A (NM_021078) Human Over-expression Lysate
SKU/Catalog Number
LS002648
Size
100 µg
Description
Transient overexpression lysate of K(lysine) acetyltransferase 2A (KAT2A)
Storage & Handling
Cite This Product
KAT2A (NM_021078) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS002648)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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KAT2A Human Over-expression Lysates NM_021078.
Western blot validation of overexpression lysate (LS002648) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KAT2A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for KAT2A (Source: Uniprot.org, NCBI)
Gene Name
KAT2A
Full Name
Histone acetyltransferase KAT2A
Weight
93.926kDa
Superfamily
acetyltransferase family
Alternative Names
EC 2.3.1.48; GCN5 general control of amino-acid synthesis 5-like 2 (yeast); GCN5L2GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2; GCN5MGC102791; General control of amino acid synthesis protein 5-like 2; General control of amino acid synthesis, yeast, homolog-like 2; hGCN5; Histone acetyltransferase GCN5; histone acetyltransferase KAT2A; hsGCN5; K(lysine) acetyltransferase 2A; Lysine acetyltransferase 2A; PCAF-b; STAF97 KAT2A GCN5, GCN5L2, PCAF-b, hGCN5 lysine acetyltransferase 2A histone acetyltransferase KAT2A|GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2|General control of amino acid synthesis, yeast, homolog-like 2|K(lysine) acetyltransferase 2A|STAF97|general control of amino acid synthesis protein 5-like 2|histone acetyltransferase GCN5|histone glutaryltransferase KAT2A|histone succinyltransferase KAT2A|hsGCN5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on KAT2A, check out the KAT2A Infographic
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In this infographic, you will see the following information for KAT2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For KAT2A (NM_021078) Human Over-expression Lysate (LS002648)
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